目的:探讨射频辅助肝切除术治疗原发性肝癌对癌细胞凋亡及正常肝脏组织损伤的影响.方法:收集在本院进行手术治疗的原发性肝癌50例,回顾其手术方式及检查结果,分为接受单纯肝切除术治疗的对照组30例、接受射频辅助肝切除术治疗的观察组20例.留取术中肿瘤组织,采用荧光定量PCR检测其中增殖基因、凋亡基因mRNA表达量;术后3 d,采集血清并采用免疫散射比浊法检测血清肝酶指标含量.结果:观察组肝癌组织中IFITM3、NCX1、CXCR2、Cep55、Bcl-2的mRNA表达量低于对照组,Caspase-3、Cyt-C的mRNA表达量高于对照组(P<0.05);术后3 d,观察组血清中肝酶指标ALT、AKP、GGT的含量与对照组相比差异不显著(P>0.05).结论:射频辅助肝切除术可有效诱导肝癌细胞凋亡,且不会增加正常肝脏组织的损伤.%Objective: To study the effect of radiofrequency-assisted hepatectomy treatment of primary liver cancer on cancer cell apoptosis and normal liver tissue damage.Methods: A total of 50 patients with primary liver cancer who received surgery in our hospital between May 2011 and August 2015 were collected, their operation methods and test results were reviewed, and then they were divided into the control group (n=30) who received hepatectomy treatment alone and the observation group (n=20) who received radiofrequency-assisted hepatectomy treatment.Intraoperative tumor tissue was collected to detect the proliferation and apoptosis gene mRNA expression by fluorescent quantitative PCR;3 d after operation, serum was collected to detect serum liver enzyme index levels by immune scatter turbidimetry.Results: IFITM3, NCX1, CXCR2, Cep55 and Bcl-2 mRNA expression in liver cancer tissue of observation group were lower than those of control group while Caspase-3 and Cyt-C mRNA expression were higher than those of control group (P<0.05);3 d after operation, serum liver enzyme indexes ALT, AKP and GGT levels of observation group were not significantly different from those of control group (P>0.05).Conclusions: Radiofrequency-assisted hepatectomy can effectively induce liver cancer cell apoptosis without increasing the normal liver tissue damage.
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