目的:探讨妊娠期高血压疾病胎儿心室收缩功能的超声评价及其与胎盘缺氧相关基因表达的相关性.方法:选择本院收治的孕晚期妊娠期高血压疾病妇女98例作为妊娠期高血压疾病组,同期在本院进行体检的孕晚期正常孕妇100例作为正常对照组.检测两组孕妇产前胎儿心室收缩功能的超声参数水平,采用荧光定量PCR法检测胎盘组织中缺氧相关基因表达量.采用Pearson检验评估妊娠期高血压疾病胎儿心室收缩功能参数水平与胎盘缺氧的相关关系.结果:妊娠期高血压疾病组胎儿左心室短轴缩短分数、右心室短轴缩短分数水平低于正常对照组胎儿.妊娠期高血压疾病组胎盘组织中血管新生相关基因VEGF、netrin-1、XIAP mRNA的表达量低于正常对照组,HIF-1α mRNA的表达量高于正常对照组胎盘组织;胎盘组织中氧化应激相关基因Nrf2、TAC3、PrxⅡmRNA的表达量低于正常对照组胎盘组织;胎盘组织中凋亡基因Fas、p53、caspase-9 mRNA的表达量高于正常对照组胎盘组织,Bcl-2、SFRP2 mRNA的表达量低于正常对照组胎盘组织.Pearson检验发现,妊娠期高血压疾病胎儿心室收缩功能超声参数水平与胎盘缺氧程度直接相关.结论:超声检测妊娠期高血压疾病胎儿心室收缩功能,可作为衡量胎盘缺氧程度的可靠手段.%Objective:To discuss the ultrasonic evaluation of fetal ventricular systolic function in hypertensive disorder complicating pregnancy and its correlation with the expression of placental hypoxia-related genes.Methods:98 late pregnant women with hypertensive disorder complicating pregnancy who were treated in the hospital between December 2014 and February 2017 were selected as hypertensive disorder complicating pregnancy group and 100 normal late pregnant women who received physical examination in the hospital during the same period were selected as normal control group.The ultrasonic parameters of fetal ventricular systolic function in two groups of pregnant women were detected before delivery,and the expression of hypoxia-related genes in placental tissue was detected by fluorescence quantitative PCR.Pearson test was used to assess the correlation between the parameters of fetal ventricular systolic function and placental hypoxia in hypertensive disorder complicating pregnancy.Results:The fetal left ventricular fractional shortening and right ventricular fractional shortening levels of hypertensive disorder complicating pregnancy group were lower than those of normal control group.Angiogenesis-related genes VEGF,netrin-1 and XIAP mRNA expression in placental tissue of hypertensive disorder complicating pregnancy group were lower than those in placental tissue of normal control group while HIF-1α mRNA expression was higher than that in placental tissue of normal control group;oxidative stress-related genes Nrf2,TAC3 and Prx Ⅱ mRNA expression in placental tissue were lower than those in placental tissue of normal control group;apoptosis genes Fas,p53 and caspase-9 mRNA expression in placental tissue were higher than those in placental tissue of normal control group while Bcl 2 and SFRP2 mRNA expression were lower than those in placental tissue of normal control group.Pearson test showed that the ultrasonic parameter levels of fetal ventricular systolic function in hypertensive disorder complicating pregnancy were directly correlated with the degree of placental hypoxia.Conclusion:Ultrasonic testing of fetal ventricular systolic function in hypertensive disorder complicating pregnancy can be used as a reliable method to measure the degree of placental hypoxia.
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