目的:研究抗H PV生物蛋白敷料联合干扰素α-2b治疗对宫颈上皮内瘤变(CIN)Ⅲ合并高危型H PV阳性患者恶性分子表达的影响.方法:选择2O14年6月~2O17年2月在32O1医院诊断为CINⅢ且高危型H PV阳性的患者,拟行锥切手术,随机分为术前接受抗 H PV生物蛋白敷料联合干扰素α-2b治疗的观察组和不进行特殊处理的对照组.取CIN病灶并测定促增殖分子、促凋亡分子、上皮间质转化分子的表达量.结果:观察组患者宫颈上皮内瘤变病灶中Rsf1、Piw il2、T O PK、p38M A PK、ERK、Snail、Twist、N-cadherin、Vimentin的mRNA表达量显著低于对照组,LRIG3、SARI、IEX-1、FHIT、E-cadherin的mRNA表达量显著高于对照组.结论:抗HPV生物蛋白敷料联合干扰素α-2b用于CINⅢ合并高危型H PV阳性患者的治疗能够抑制肿瘤细胞的增殖及浸润性生长.%Objective:To study the effects of anti-HPV bioprotein dressing combined with interferon α-2b therapy on the malignant molecule expression in patients with cervical intraepithelial neoplasia(CIN) Ⅲ complicated by high-risk HPV positive. Methods:Patients who were diagnosed with CIN Ⅲ and high-risk HPV positive and underwent conization in the 32O1 Hospital Affiliated to Xi'an Jiaotong University between June 2O14 and February 2O17 were selected and randomly divided into the observation group who received preoperative anti-HPV bioprotein dressing combined with interferon α-2b therapy and the control group who received no special treatment. CIN lesion was collected to determine the expression of pro-proliferation mol-ecules,pro-apoptosis molecules and epithelial-mesenchymal transition molecules. Results:Rsf1,Piwil2,TOPK,p38MAPK, ERK,Snail,Twist,N-cadherin and Vimentin mRNA expression in cervical intraepithelial neoplasia lesions of observation group were greatly lower than those of control group whereas LRIG3,SARI,IEX-1,FHIT and E-cadherin mRNA expression were greatly higher than those of control group. Conclusion:Anti-HPV bioprotein dressing combined with interferon α-2b ther-apy can inhibit the proliferation and invasive growth of tumor cells in patients with CIN Ⅲ complicated by high-risk HPV posi-tive.
展开▼
