目的:研究神经母细胞瘤中KLF4、UBE2C表达水平与细胞黏附、迁移的相关性.方法:选择2014年5月~2017年2月期间在恩施土家族苗族自治州中心医院诊断为神经母细胞瘤的56例患儿作为研究的NB组并收集病灶组织,选择同期因严重肾盂积水在恩施土家族苗族自治州中心医院就诊的38例患儿作为研究的对照组并收集正常肾上腺组织.测定临床组织样本中KLF4、UBE2C的mRNA表达量及蛋白表达量以及细胞黏附分子、迁移分子的蛋白表达量.结果:NB组神经母细胞瘤组织内KLF4的mRNA表达量及蛋白表达量均显著低于对照组,UBE2C的mRNA表达量及蛋白表达量均显著高于对照组;NB组神经母细胞瘤组织内 PDLIM1、AMF、GPx1、L1CAM、Nrg1、RANK、RANKL、Inβ1、MTA1、MMP9的蛋白表达量均显著高于对照组且与KLF4的蛋白表达量呈负相关,与 UBE2C的蛋白表达量呈正相关.结论:神经母细胞瘤中低表达的KLF4以及高表达的UBE2C能够促进肿瘤细胞的黏附和迁移.%Objective:To study the correlation of KLF4 and UBE2C expression levels in neuroblastoma with cell adhe-sion and migration.Methods:56 children who were diagnosed with neuroblastoma in the Central Hospital of Enshi Autonomous Prefecture between May 2014 and February 2017 were selected as the NB group of the study,and the lesion tissue was collect-ed;38 children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to serious hydronephrosis during the same period were selected as the control group of the study,and the normal adrenal gland tissue was collected.The mRNA expression and protein expression of KLF4 and UBE2C as well as the protein expression of cell adhesion molecules and migra-tion molecules in clinical tissue samples were determined.Results:The mRNA expression and protein expression of KLF4 in neuroblastoma tissue of NB group were greatly lower than those of control group whereas the mRNA expression and protein expression of UBE2C were greatly higher than those of control group;PDLIM1,AMF,GPx1,L1CAM,Nrg1,RANK, RANKL,Inβ1,MTA1 and MMP9 protein expression in neuroblastoma tissue of NB group were greatly higher than those of control group,negatively correlated with the protein expression KLF4,and positively correlated with the protein expression of UBE2C.Conclusions:The low expression of KLF4 and the high expression of UBE2C in neuroblastoma can promote the adhe-sion and migration of tumor cells.
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