目的：：研究分析了胃癌组织中 miRNAs 及下游靶基因的表达量及其与临床病理分期的关系。方法：选择在我院确诊为胃癌的患者进行研究,收集胃癌组织和癌旁组织,测定 miRNAs 的表达量以及耐药相关基因、增殖相关基因、EMT 相关基因所编码蛋白的含量。结果：胃癌组织中 miR-21、miR-106a 、miR-192、miR-194、miR-210、miR-215的表达显著上调,miR-30a 、miR-125、miR-149、miR-194、miR-205、miR-365的表达显著下调且肿瘤的 TNM 分期越高,上述 miRNAs 表达的变化越明显；miR106和 miR-30a 的变化趋势最明显,前者上调4.38倍、后者下调0.23倍；胃癌组织中 P-gP 、GST-π、CACNA2D1、RPL23、H sp27、ZNF139、Mcmp4、OPCML、N-cadherin、Vimentin 的含量明显高于癌旁组织,E-cadherin 的含量明显低于癌旁组织；miR106表达量与 P-gP 、GST-π、CACNA2D1、RPL23、H sp27、ZNF139、Mcmp4、OPCML、N-cadherin、Vimentin 的含量呈正相关,与 E-cadherin 的含量呈负相关；miR-30a 表达量与 P-gP 、GST-π、CACNA2D1、RPL23、H sp27、ZNF139、Mcmp4、OPCML、N-cadherin、Vimentin 的含量呈负相关,与 E-cadherin 的含量呈正相关。结论：胃癌组织中 miR106的表达明显增加、miR-30a 的明显减少,miR106和 miR-30a 能够调控耐药基因、增殖基因以及 EMT 基因的表达。%[ABSTRACT]Objective:To study and analyze the expression of miRNAs and downstream target genes in gastric cancer tissue and its relationship with clinicopathologic stage.Methods:Patients diagnosed with gastric cancer in our hospital were se-lected for study,and gastric cancer tissue and paracancer tissue were collected to detect the expression of miRNAs as well as the contents of proteins encoded by drug resistance-related genes,proliferation-related genes and epithelial-mesenchymal transi-tion (EMT)-related genes.Results:The expression of miR-21,miR-106a,miR-1 92,miR-1 94,miR-210 and miR-21 5 in gas-tric cancer tissue was significantly up-regulated,while expression of miR-30a,miR-125,miR-149,miR-1 94,miR-205 and miR-365 was significantly down-regulated,and the higher the TNM stage of tumor is,the more significant the change of the expression of above miRNAs becomes;the changing trend of miR106 and miR-30a was the most significant,with that the for-mer was up-regulated by 4.38 times and the latter was down-regulated by 0.23 times;the contents of P-gP,GST-π,CAC-NA2D1,RPL23,Hsp27,ZNF139,Mcmp4,OPCML,N-cadherin and Vimentin contents in gastric cancer tissue were signifi-cantly higher than those in paracancer tissue,and E-cadherin content was significantly lower than that in paracancer tissue;miR106 expression level was positively correlated with contents of P-gP,GST-π,CACNA2D1,RPL23,Hsp27,ZNF139,Mc-mp4,OPCML,N-cadherin and Vimentin and negatively correlated with E-cadherin content;miR-30a expression level was neg-atively correlated with contents of P-gP,GST-π,CACNA2D1,RPL23,Hsp27,ZNF139,Mcmp4,OPCML,N-cadherin and Vimentin and positively correlated with E-cadherin content.Conclusions:The miR106 expression significantly increases and miR-30a expression significantly decreases in gastric cancer tissue,and miR106 and miR-30a can regulate the expression of drug resistance genes,proliferation genes and EMT genes.
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