建立龙胆HPLC指纹图谱研究方法，对不同产地龙胆的质量进行研究．色谱柱：Spherisorb－C18（4．6mm ×250 mm，5μm），水（A）－甲醇（B）进行梯度洗脱；流速为1．0 mL／min；检测波长为270 nm；柱温为30℃；进样量为10μL；检测时间为40 min．结果表明，东北三省不同产地龙胆的指纹图谱没有明显差异，但不同产地的龙胆苦苷的含量存在明显的差异．该色谱条件简单易行，可用于龙胆的鉴别研究．%To establish the method of fingerprint analysis of Gentian chinensis by HPLC , and study the quality of Gentian chinensis from different habitats .The chromatographic condi-tions:chromatographic column: Spherisorb -C18 (4.6 ×250 mm, 5 μm), mobile phase:water(A), methanol(B);flow rate:1.0 mL/min;detection wavelength:270 nm;column temperature:30 ℃;sample size:10μL;measurement time:40 min.The results indicated that there were no particularly significant differences between fingerprints of Gentian chinen -sis from different habitats .But the content of different habitats of Gentiopicroside exists obvi-ous differences .This chromatographic condition was simple and could be used for identifica-tion of Gentian chinensis .
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