Objective To compare the effects of salmon calcitonin (CT) and parathyroid hormone(PTH) (1-34) on bone mineral density and bone histomorphometry of vertebra in ovariectomized rats.Methods Forty female Sprague-Dawley rats aged 3 months were randomly divided into four groups(with 10 rats each).All rats but those in the Sham group received ovariectomy and treated by vehicle (OVX),salmon calcitonin (OVX+ CT) and PTH (1-34) (OVX +PTH).After animals were sacrificed at 6 months post-OVX,the L4-5 spinal segments were harvested.Bone mineral density (BMD) and histomorphometry analysis were performed to evaluate the bone mass and microstructural changes in the lumbar vertebral bodies.Histological analysis with Gemisa staining was used to identify the characteristics of the degenerative discs.Results Six months after OVX,the BMD values of L4 vertebral bodies in the OVX group were significantly deccreased,compared with the Sham group(P＜0.05).The BMD values in the OVX+CT group and OVX+PTH group were significantly increased,compared with the OVX group(P＜ 0.05),and the BMD values in the OVX+PTH group were significantly increased than that in the OVX+CT group(P＜0.05).The BV/TV values of L5 vertebral bodies in the OVX group were significantly decreased,compared with the Sham group,the BV/TV values in the OVX+CT group were significantly increased than that in the OVX group,the BV/TV values in the OVX+ PTH group were significantly increased,compared with the Sham,OVX and OVX+CT group (P＜0.05).The Tb.Th values in the OVX group and OVX+CT group were significantly decreased,compared with the Sham group,the Tb.Th values in the OVX+PTH group were significantly increased than that in the Sham group,OVX group and OVX+CT group(P＜ 0.05).The values of Tb.Sp in the OVX group were higher than that in the Sham group,OVX+ CT group and OVX+PTH group(P＜0.05).There are no significantly changes of Tb.N values in each groups(P＞0.05).Conclusion Both salmon calcitonin and parathyroid hormone(1-34) can improve the bone mass and suppress the high bone turn-over in OVX rats.But the effect of parathyroid hormone(1-34) to suppress the bone resorption and improve the bone mass is better than that of salmon calcitonin.%目的 比较鲑鱼降钙素(salmon calcitonin,CT)及甲状旁腺素(parathyroid hormone,PTH)(1-34)对卵巢切除大鼠腰椎骨密度(bone mineral density,BMD)及骨组织形态计量学的影响.方法 40只3月龄雌性Sprague-Dawley(SD)大鼠,随机分为4组,每组10只:假手术(Sham)组;双侧卵巢切除(OVX)组;双侧卵巢切除+甲状旁腺素(1-34)(OVX+ PTH)组;双侧7卵巢切除+鲑鱼降钙素(OVX+CT)组.OVX+CT组及OVX+ PTH组于卵巢切除术后3个月分别给予皮下注射CT或PTH(1-34).连续给药3个月后处死所有大鼠并收集标本.取L4椎体进行BMD检测.L5椎体进行Gemisa染色及骨组织形态计量学检测.结果 卵巢切除术后6个月,OVX组BMD显著低于Sham组(P＜0.05),而OVX+ CT组及OVX+ PTH组椎体BMD显著高于OVX组(P＜0.05),且OVX+PTH组BMD显著高于OVX+ CT组(P＜0.05).OVX组BV/TV低于Sham组,OVX+ CT组高于OVX组,OVX+ PTH组BV/TV高于Sham组、OVX组、OVX+ CT组,差异均有统计学意义(P＜0.05).OVX组、OVX+ CT组Tb.Th低于Sham组,OVX+ PTH组高于Sham组、OVX组、OVX+CT组,差异均有统计学意义(P＜0.05).OVX组Tb.Sp高于Sham组,OVX+ CT组和OVX+PTH组低于OVX组,差异均有统计学意义(P＜0.05).各组大鼠Tb.N差异无统计学意义(P＞0.05).结论 CT及PTH(1-34)可显著增加卵巢切除大鼠腰椎BMD并抑制骨重建的高转换状态,且PTH(1-34)作用要优于CT.
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