以刺槐(Robinia pseudoacacia)与皂荚(Gleditsia sinensis)的1年生苗为材料,通过盆栽控水试验,研究干旱胁迫对2种苗木叶片中渗透调节物质含量、丙二醛(MDA)含量及保护酶活性的影响.结果表明:随着干旱胁迫强度的增大,2种苗木叶片的脯氨酸(Pro)含量、可溶性糖含量比正常水分时升高,相同水分梯度下刺槐Pro、可溶性糖含量升高幅度较大;皂荚叶片中可溶性蛋白含量增加,刺槐叶片中可溶性蛋白含量降低;MDA含量随水分胁迫程度的加剧均表现出升高趋势,且皂荚在相同水分梯度下增幅较大;超氧化物歧化酶(SOD)活性增加,但刺槐增加幅度大于皂荚;皂荚叶片中过氧化物酶(POD)活性增加、过氧化氢酶(CAT)活性降低,而刺槐叶片中CAT活性增加、POD活性降低.可知,2种苗木通过渗透调节物质的积累、抗氧化酶活性的升高来增强对干旱胁迫的适应性.总体来看,刺槐耐干旱能力大于皂荚.%This paper studied the effects of drought stress on the content of osmotic adjustment substances,MDA and protective enzyme activity in the leaves of one-year-old Gleditsia sinensis and Robinia pseudoacacia through the experiment of controlling water by potted planting.The results indicated that with the aggravation of drought stress,the content of Pro and soluble sugar in the leaves of the two seedlings were higher than that of normal water,Robinia pseudoacacia had greater increasing amplitude under the same water gradient.The content of soluble protein in the leaves of Gleditsia sinensis increased,but in the leaves of Robinia pseudoacacia decreased.The content of MDA increased with the aggravation of water stress,but Gleditsia sinensis increased significantly under the same water gradient.The activity of SOD increased,however the increasing amplitude of Robinia pseudoacacia ＞ Gleditsia sinensis.POD activity increased and CAT activity decreased in leaves of Gleditsia sinensis,while the Robinia pseudoacacia leaves were just the opposite.The results showed that the two seedlings enhanced the adaptability of drought stress through the accumulation of osmotic adjustment substance and the increase of antioxidant enzyme activity.Overall,the tolerance to drought of Robinia pseudoacacia ＞ Gleditsia sinensis.
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