There are serious browning and low induction rate when the protocorn-like body (PLB) was induced from leaves of Phalaenopsis amabilis. Using the grem free leaf of Phalaenopsis ama-bilis H807 as materials, the effects of anti-browning materials(citric acid, ascorbic acid and activated charcoal)and cytokinins (6-BA and TDZ) on the protocorn-like body induction was investigated. The results indicated that the rank of anti-browning only reached the level 1 with 70 mg/L ascorbic acid,which was best to prevent in vitro leaves from browning. The protocorn-like body induction rate reached 75. 0% with 3 mg/L TDZ. On the medium with 1/2MS+70 mg/L ascorbic acid,hormone combination of 3 mg/L TDZ+1 mg/L NAA was the best for PLB induction.%以蝴蝶兰杂交品种H807无菌苗的叶片为材料,研究了防褐化物质(柠檬酸、抗坏血酸及活性炭)和细胞分裂素(6 - BA与TDZ)对类原球茎诱导的影响.结果表明:在添加70 mg/L抗坏血酸的培养基中,叶片褐化级别仅为1级,防褐化效果最佳；3 mg/L的TDZ对蝴蝶兰叶片诱导类原球茎的诱导率最高,为75.0％.在以1/2MS附加70 mg/L抗坏血酸的基础上,激素组合TDZ3 mg/L+NAA 1 mg/L对蝴蝶兰H807类原球茎的诱导效果最佳.
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