The genetic diversity of the CDS of Hsf2 gene from 14 species, including Homo sapiens ,Pan troglodytes ,Nomascus leucogency ,Macaca mulatta ,Callithrix jacchus ,Oryctolagus cuniculus, Ailuropoda melanoleuca ,Sus scrofa ,Bos Taurus ,Loxodonta Africana ,Rattus norveg-icus ,Mus musculus , Cavia porcellus and Cricetulus qriseus were analyzed using the method of comparative genomics' and bioinformatics. The characteristics of composition of nucleic acid sequences and amino acid sequences were also analyzed. The results showed that a total of 203 polymorphic sites, including 68 single polymorphic sites, the percentage was about 9. 81%, that could be sorted into 17 haplotypes were detected from 40 sequences of 14 species. Comparing with its CDS and the structure of protein conservative within species, it comes to relative diversity in Hsf2 for nucleotide among species. The amino acid sequences of Hsf2 presented hydrophilic,theoretical PI was less than 7 mostly. The nature of polypeptide was acid and the instability index of polypeptide was between 51. 90 and 58. 13,indicating that the polypeptide was not stable.%采用比较基因组学和生物信息学方法,分析了人、黑猩猩、长臂猿、猕猴、狨、欧洲兔、大熊猫、野猪、牛、非洲象、褐家鼠、小家鼠、荷兰猪和灰仓鼠共14个物种的热休克转录因子2(或称热激因子2,heat shock transcription factor 2,HSF2)基因编码区(CDS)的遗传多样性,并对该基因的氨基酸序列、组成成分等进行预测和推断.结果表明,在来自14个物种的40条基因序列中共检测到多态位点数203个,其中有单一多态位点68个,百分率约为9.81％,检测到单体型17种,Hsf2基因CDS在种内表现较为保守,种间则表现有较丰富的遗传多样性.Hsf2的氨基酸序列表现为亲水性,理论等电点大多小于7,表现为酸性；肽链的不稳定系数在51.90～58.13,表明多肽不稳定.
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