目的:研究氟他胺致小分子热休克蛋白基因(sHsps)在小鼠阴茎组织中的表达改变.方法:ICR小鼠受孕后,将其随机分为实验、对照两组,实验组和对照组各16只,于孕12d(GD12)-GD19,每天经口给予实验组孕鼠混悬于玉米油中的氟他胺80 mg/kg/day,对照组孕鼠给予等体积的玉米油.分别于出生后第7、21天(PND7、PND21)在对照组、实验组随机取8窝子鼠,并在对照组随机选取雄性小鼠1只,实验组选取肛门与生殖器之间的距离(AGD)正常和异常雄性小鼠各1只.取上述雄性子鼠的阴茎,利用实时荧光定量PCR相对定量检测阴茎组织中sHsps的表达丰度.结果:无论是实验组还是对照组,sHsps在PND7、PND21的小鼠阴茎组织中都有表达.除Hspb9在PND7实验组AGD"正常"雄性子鼠阴茎中的表达和对照组无差异外,Hsp27、Hspb3、Hspb5、Hsp20、Hspb9在实验组PND7、PND21中的表达均高于对照组.实验组Hsp25、Hspb2、Hspb4、Hspb10、HO-1在PND7、PND21的表达均低于对照组.实验组Hsp22在PND7的表达和对照组无差异,但在PND21的表达则高于对照组.Hspb7在PND7实验组中的表达高于对照组,而在PND21实验组中的表达则低于对照组.结论:Hsp25、Hspb2、Hspb4、Hspb10、HO-1可能和小鼠阴茎异常发生有关,Hspb7可能是小鼠阴茎发育中起重要作用的基因,而Hsp27、Hspb3、Hspb5、Hsp20、Hspb9和Hsp22在小鼠阴茎发育时可能起应激保护作用.%Objective To study the expression of sHsps in mouse penis with hypospadias induced by flutamide. Methods ICR pregnant mice were divided randomly into the treatment and the control groups, which were 16 for each. Flutamide sus-pension in maize oil was administered per os to the treatment group on 12d (gestational day 12, GD12) -GD19 at a daily dose of 80mg/kg/day, whereas the same dose of maize oil was fed to the control group. The male offspring of 8 dams were selected on postnatal 7 and 21 days (PND7, PND21) in both groups respectively. And one of them of the different mothers in the control group was picked out randomly, while twowere selected from the treatment group randomly, for one wasnormal anogenital dis-tance (AGD) and another abnormal AGD. And furthermore, the relative expression abundance of the sHsps of all the samples was measured by real-time quantity reverse transcript polymerase chain reaction (qPCR). Results In both groups, sHsps were found expressed in the mice penises on PND7 and PND21. Except there was no difference of the expression of Hspb9 between the AGD"normal"male offspring in the treatment group and that of the control group on PND7, expression of Hsp27, Hspb3, Hspb5, Hsp20andHspb9 in the treatment group on PND7 and PND21 was higher than that of the control group. The expression of Hsp25, Hspb2, Hspb4, Hspb10 and HO-1 on PND7 and PND21 in the treatment group was lower than that in the control group. The expression of Hsp22 in both groupshad no difference on PND7, but it was higher in the treatment group than the control group on PND21. The expression of Hspb7 in the treatment group on PND7 was higher than that in the control group, while lower on PND21. Conclusion During mouse penisdeveloping, Hsp25, Hspb2, Hspb4, Hspb10 and HO-1 may be related to the abnormal development, and Hspb7was possibly a gene with important role, whileHsp27, Hspb3, Hspb5, Hsp20, Hspb9 and Hsp22 maybe played a protective effect.
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