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湖南师范大学学报（医学版）

湖南师范大学学报（医学版）

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Journal of Hunan Normal University(Medical Science)
《湖南师范大学学报(医学版)》为湖南师范大学主办的医药卫生类综合性学术期刊，创刊于2004年，2010年11月被收录为“中国科技核心统计源期刊”。本刊宗旨为反映科研、医药新成果、新技术、新经验，报道国内外医学新动态，促进学术交流，提高学术水平，推动科技进步。欢迎广大医药卫生人员及医学工作者来稿。 　　2004年经国家新闻出版总署批准，创办《湖南师范大学学报(医学版)》，由湖南省教育厅主管，湖南师范大学主办，湖南师范大学医学院承办的国内外公开发行的医药卫生类综合性学术期刊。自创刊起，在学校的大力支持下，医学院高度重视，对学报的办刊宗旨、定位及怎样办成精品等进行了认真的研究。《湖南师范大学学报(医学版)》为高校、科研院所和大型医院的医学工作者提供新的平台，以反映医药领域科研新成果、新技术、新经验为主，报道国内外医学发展新动态，促进学术交流，提高学术水平，推动科技进步。学报设置了基础医学、临床医学、药学、预防医学等栏目。在办刊过程中，坚持科学性、创新性的原则和严谨、求实的态度，从高起点严格要求，认真把握审稿、定稿、编辑、排版各个环节，力求使学报办成学术水平高、严谨规范、可读性强和具有一定特色的期刊，通过多年的努力，学报质量逐年提升，影响力逐步扩大。近日获悉，经过多项学术指标综合评定及同行专家评议推荐，于2010年11月收录为“中国科技核心统计源期刊”，并由中国科学技术信息研究所颁发了证书。中国科学技术信息研究所出版的“中国科技论文统计源期刊”，又称“中国科技核心期刊”，学科范畴主要为自然科学领域，是目前国内公认的科技统计源期刊目录，权威性名列国内首位。
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  • 被引量：2967
  • H指数：1
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  • 国内刊号/CN：43-1449/R
    国际刊号/ISSN：1673-016X
  • 发行周期：季刊
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  • 主管单位：湖南省教育厅
    主办单位：湖南师范大学
湖南师范大学学报（医学版）-联系信息
  • 主编：符晓华
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  • 邮箱：hnnms@126.com
  • 地址：湖南省长沙市岳麓区咸嘉湖1号
  • 邮编：410006
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  • 摘要：目的：研究Antiflammin-1(AF-1)与其受体在活细胞表面的结合特性。方法：激光共聚焦显微镜观察标记的AF-1与NIH 3T3细胞的结合；基于流式细胞分析的配基结合方法对不同方法标记的AF-1与细胞表面的受体结合动力学参数进行分析。结果：激光共聚焦显微镜观察提供了AF-1与活细胞结合的证据；流式细胞分析结果表明，过量未标记的AF-1可以竞争标记的AF-1与细胞表面的结合。 Scatchard作图为线性，符合简单位点系统特征，Kd值在29.19~46.33nM之间，最大结合容量Bmax为93.294.267 RFI。结论：AF-1与NIH 3T3在活细胞表面呈高亲和力结合。本研究结果有助于揭示 AF-1与其受体在生理条件下的结合特性。%Objective To determine the binding characteristics of antiflammin-1 (AF-1) to its receptor on the surface of living cells. Methods Confocal microscope was used to examine the binding of labeled AF-1 to NIH 3T3 cells. Flow cytometry-based binding assays were used to determine the binding kinetics of AF-1 to cell surface. Results Confocal microscope provided visual evidence that AF-1 bind to living cells. Flow cytometry-based binding assays showed that excess unlabeled peptide AF-1 displaced labeled AF-1 binding with cell sur-face. Scatchard analysis revealed a liner plot, suggesting a single population of binding sites with a dissociation constant (Kd value) of 37.75±4.003 nmol/L (Bmax=93.29±4.267 RFI). Conclusion These data indicated that AF-1 bound with high affinity to NIH 3T3 cell surface in living cells, which helped in understanding the binding pa-rameters of AF-1 to its receptor under physiological conditions.
  • 摘要：目的：探讨伴频发室早高血压病患者射频消融术后心功能的变化方法：收集2009年1月~2011年3月间在我院就诊并行射频消融治疗的频发室早患者38例，其中21例特发性室早患者作为对照组，17例伴有频发室早高血压病患者列为病例组；所有患者分别于术前及术后3月、6月、12月采用二维心脏超声测绘左室舒张末期内径（LVEDD）及血清氨基-末端脑钠肽（NT-proBNP）水平。结果：各组术后3月NT-proBNP值分别为：140.4pg/ml及153.1pg/ml，较术前明显下降（P＜0.05）；但术后12月与3月比较差异无统计学意义。各组术后3月LVEDD较术前无明显变化；术后6月两组LVEDD值分别为：（47.2±3.1）mm 、（46.7±2.8）mm，各自与术前比较，差异有统计学意义（P＜0.01）；术后12月与6月比较LVEDD进一步缩小，但两者比较差异无统计学意义。组间NT-proBNP及LVEDD各自同期比较差异不明显。结论：伴频发室早高血压病患者行射频消融治疗心脏结构和心功能恢复较好，可尝试在临床上推广运用。%Objective The purpose of this study was to investigate the changes in heart function of radiofrequen-cy catheter ablation in hypertensive patients with frequent PVC. Methods Thirty-eight consecutive patients undergo-ing Ea-guided radiofrequency catheter ablation (RFCA) were studied of whom twenty-one consective patients had Id-iopathic frequent PVC(as control group);seventeen hypertensive patients with frequent pvc(as case group). Echocar-diographic measurements of Left ventricular end-diastolic diameter size (LVEDD) and measures of the serum N-ter-minal brain natriuretic peptide (NT-proBNP) level of all the patients were obtained at baseline and after 3 months, 6 months, 12 months of RFCA. Results The NT-proBNP level of the two groups after 3 months of RFCA was significant-ly lower than basic level, respectively,( case group:153.1 vs 195.7pg/ml, P<0.01;contral goup:140.4 vs 180.3 pg/ml, P<0.05). But NT-proBNP level was no significant difference between the 12 months and 3 months after RFCA.In each groups, the LVEDD was no significant difference between the baseline and the 3 months after RFCA;LVEDD of 6 months after RFCA was much smaller than basic line in each group (case group:46.7±2.8 mm vs 52.9±3.8mm, P<0.01;control group:47.2±3.1mm vs 53.0±4.2mm, P<0.01).The LVEDD showed downward trend compared with the 12-month to 6 moths affter RFCA;but had no significant difference between them.NT-proBNP level and LVEDD was no notable difference btween the two groups at the same period, respectively. Conclusion It is effective of RFCA in hypertensive patients with frequent PVC,so can be extensivly used in clinical.
  • 摘要：目的：本研究检测表皮生长因子样域7（EGFL）在胃癌中的表达并探讨其临床意义。方法：收集45例新鲜胃癌，用免疫组化SP法检测EGFL7的表达，分析其表达与肿瘤分级及微血管密度（MVD）的相关性。结果：有淋巴结转移组EGFL7阳性率高于无淋巴结转移组，浸润浆膜层组阳性率高于未浸润浆膜层组。癌组织中EGFL7的表达与淋巴结转移及浸润深度呈正相关（P<0.05）。胃癌低分化组MVD高于高分化组和中分化组；浸润至浆膜层组MVD高于未至浆膜层组；有淋巴结转移组MVD高于无淋巴结转移组。癌组织中MVD与分化程度、浸润深度及有无淋巴结转移呈正相关(P<0.05)。 EGFL7表达阳性的肿瘤组织中MVD均值33.80±10.56，高于EGFL7阴性表达组26.00±7.21（P<0.05），采用Pearson 进行相关分析，发现MVD 值与EGFL7表达正相关（r=0.313,P<0.05）。结论：EGFL7是一个新的预测胃癌临床进展的标记物，并可能成为胃癌患者抗血管新生的治疗靶点。%Objective In the current study, we examined the expression of EGF-like domain 7 (EGFL7), an endothelial cell-derived secreted factor, in gastric carcinoma and explored its clinical significance. Methods The expression of EGFL7 protein and MVD in 45 cases gastric carcinoma were detected by immunohistochemical methods.We also analyzed the correlations between the expression levels of EGFL7 and various clinical parame-ters, including tumor grade, tumor proliferation marker FⅧ, and microvessel density (MVD).Result The expres-sion of EGFL7 in the team which has lymph node metastasis is higher than the team which hasn't ,in the serosal invasion team is higher than the team which doesn't have serosal invasion. The expression of EGFL7 in gastric carcinoma is related with lymph node metastasis and tumor invasion (P<0.05).The MVD of poorly differentiation team is higher than the well-differentiation team and differentiated team. The serosal invasion team's MVD is higher than the team which is not,the team's MVD which has lymph node metastasis is higher than the team which hasn't.The MVD is related with tumor differentiation, invasion and lymph node metastasis(P<0.05).MVD in the tumor which express EGFL7 is 33.80±10.56, that is higher than the MVD in the tumor which don't express (26.00±7.21)(P<0.05).Pearson analysis shows MVD and EGFL7 expression is positive correlation (r=0.313,P<0.05)Conclusion Our data suggest that EGFL7 expression is a novel predictive factor for the clinical progression of gastric carcinoma, and may constitute a therapeutic target for anti-angiogenesis therapy in patients with the dis-ease.
  • 摘要：Objective To investigate the effect of dual control modes of mechanical ventilation in patients with ARDS. Methods ARDS 81 cases with mechanical ventilation used volume control modes、pressure control modes and dual control modes in turn randomly. Period of time later, monitored the change of the hemodynamic、ABG and the ventilatory parameters. Results The ventilation pressure of dual control modes was less than volume control modes and close to pressure control modes. RR、MV、oxidation index (OI) and hemodynamic have no obvi-ous difference in three modes. Barotraumas didn't occur. Conclusion Dual control modes combines the character-istics of volume control mode and pressure control mode, improve the harmony between the patients and the ven-tilator. But be compared with traditional ventilation, dual control modes are in defect of predominance. None but treating with lung protective ventilatory strategies(LPV) can achieve the good ventilation effect.%　　目的：探讨应用双重控制模式(dual control modes)与容控和压控模式的机械通气在治疗急性呼吸窘迫综合症（ARDS）中的效果比较。方法：对81例ARDS病人随机分组给予容控模式(volume control mode)、压控模式(pressure control mode)以及双重控制模式通气，采用分组对照的方法监测病人机械通气参数、通气、换气和血流动力学指标的变化。结果：双重控制模式气道压力小于容控通气，与压控通气组无明显差异。RR、分钟通气量（MV）、氧合指数（OI）、血流动力学指标三种模式无明显差异。无一例出现气压伤。结论：双重控制模式结合了容控通气与压控通气的特点，提高了人机协调性，但与传统通气模式相比，治疗中没有绝对的优势。只有合理地采用个体化肺保护通气策略(LPV)，才能取得良好的通气效果。
  • 摘要：Objective To investigate BORIS protein expression in colorectal cancer and determinate its correlation with clinicopathological feature.Methods Samples of the 100 cases were divided into 2 groups:60 cas-es of colorectal cancer tissues,40 cases of incisal edge normal colorectal tissues,Immunohistochernical method was applied to detect the expression of BORIS protein in tissues.Analyzed the expression of BORIS in the tissues in group of colorectal cancer and group of normal controls. And analyzed the differential expression of BORIS in colorectal cancer and its relationship with colorectal cancer in clinical and pathological features.Results The ex-pression of BORIS in colorectal cancer was significantly higher than that of normal colorectal mucosa (P<0.05). The expression of BORIS was related to tumor size, invasive depth, Duke′s stage (C+D), lymph node metastasis, and differentiation in colorectal carcinoma (P<0.05). However, BORIS expression was not related to age, gender, and tumor site.Conclusion The high expression of BORIS protein may be playing an important role in the devel-opment process of colon cance, BORIS can be applied to the auxiliary diagnosis of large intestine cancer.%　　目的：探讨大肠癌中BORIS蛋白的表达与肿瘤病理特征的关系。方法：应用免疫组织化学方法研究（60）例大肠癌组织及其配对的（40）例切缘正常大肠组织BORIS的表达情况，比较BORIS在不同大肠组织中表达的差异及其与大肠癌病理因素之间的关系。结果：大肠癌组织中BORIS蛋白表达阳性率明显高于切缘正常大肠组织（P<0.05），BORIS蛋白表达阳性率与肿瘤大小、浸润深度、Dukes分期、淋巴结转移、分化程度有关（P<0.05)；与患者年龄、性别、肿瘤发生部位无关（P>0.05)。结论：BORIS过量表达可能在大肠癌的发生、发展过程中起重要作用，可能用于大肠癌辅助诊断。
  • 摘要：目的：7-二氟甲氧基-5,4'-二甲氧基金雀异黄素(7-difluoromethoxy-5,4'- dimethoxygenistein, dFMG)对溶血性磷脂酰胆碱(lysophosphatidylcholine, LPC)诱导人脐静脉内皮细胞（HUVE-12）损伤模型的保护作用，探讨其是否通过抑制CD40/CD40L相互作用 E-选择素的释放相关。方法：体外培养人脐静脉内皮细胞（HUVE-12）。 Annexin V/PI 染色流式细胞术（FCM）分析细胞凋亡率，用流式细胞仪检测CD40/CD40L的表达，酶联免疫吸附法检测E-选择素的释放。结果：LPC（10μmol/L）处理HUVE-12(6h、12h、24h和48h），24h的细胞凋亡率为（30.98%±2,04%）（P <0.05）。 DFMG 预孵育降低 LPC 诱导HUVE-12的细胞凋亡率，CD40/CD40L的表达降低及E-选择素的释放减少（P<0.05）。结论：DFMG 拮抗LPC 诱导HUVE-12细胞凋亡作用与减少CD40的表达及E-选择素的释放有关。%Objective To explore protected effects of 7-difluoromethoxy-5, 4'-dimethoxygenistein on Hu-man umbilical vein endothelial cells injury induced by lysophosphatidylcholine, is whether by inhibiting CD40/CD40L interaction and endothelium-selectin release. Methods HUVE-12 were cultured in vitro.The apoptotic rate was detected by flow cytometry using Annexin V/PI staining.CD40/CD40L interaction expression were deter-mined by flow cytometry.The enzymatic activity of endothelium-selectin were determined by ELISA.Results Treatment with 10 μmol/L LPC for (6h、12h、24h、48h), the apoptotic rate of HUVE-12 for 24h increased to (30.98%±2.04%)(P<0.05).The pretreatment with DFMG significantly decreased induction of Human umbilical vein endothelial cells death,the expression of CD40/CD40L interaction and the expression of endothelium-se-lectin. Conclusion Antagonistic effects of DFMG on HUVE-12 injury induced by LPC is associated with de-creased the expression of CD40/CD40L and the expression of endothelium-selectin.
  • 摘要：目的：探讨维生素E对脂多糖(lipopolysaccharide,LPS)诱导的急性肺损伤（acute lung injury,ALI）中氧化应激状态的影响。方法：以Wistar大鼠为研究对象，实验分成正常对照组、ALI组及维生素E预处理组。用LPS作用于大鼠6 h后检测各组大鼠肺组织中丙二醛（Malondialdehyde,MDA）的含量、乳酸脱氢酶（lactate dehydroge-nase,LDH）及超氧化物歧化酶（superoxide dismutase,SOD）活性，并应用RT-PCR及ELISA法检测大鼠肺组织中TNF-α的水平。结果：ALI组与正常对照组比较SOD活性下降、LDH的活性增强、MDA的含量升高，且TNF-αmRNA 及蛋白的表达增加（P﹤0.05）；维生素E预处理组与ALI组比较SOD活性增强，LDH的活性减弱， MDA的含量降低，TNF-αmRNA 及蛋白的表达降低（P﹤0.05）。结论：维生素E预处理可减轻LPS诱导的ALI大鼠肺组织的脂质过氧化损伤，减少炎症因子的表达，发挥一定的抗损伤效应。%Objective To explore the effect of vitamin E on oxidative stress of lipopolysaccharide (LPS) in-duced acute lung injury (ALI).Methods Wistar rats as the research object, the experiment is divided into normal group, ALI group and vitamin E pretreatment group. After LPS treat rats on 6 hours,MDA content, LDH and SOD activity in the lung tissue were detected, RT-PCR and ELISA were used to detect TNF-αexpression of lung.Re-sults ALI groups activity of SOD decreased, activity of LDH strengthen,content of MDA increased and TNF alpha mRNA and protein expression increased compared with normal groups (P﹤0.05).Vitamin E pretreatment groups activity of SOD strengthen, activity of LDH abated, content of MDA reduced, and TNF alpha mRNA and protein expression reduced compared with LPS groups (P﹤0.05).Conclusion Vitamin E pretreatment can reduce lipid peroxidation damage and inflammatory factor expression of lung tissue in LPS-induced ALI, playing against-in-jury effect.
  • 摘要：Objective To explore the effect on prognosis of Gynecological retroperitoneal lymphadenectomy for placing placed T tube drainage.Methods From March,2004 to April , 2010 91 patients of cervical cancer and endometrial cancer suffering from radical hysterectomy and gynecological retroperitoneal lymphadenectomy were divided into two groups: vaginal T tube drainage in A group (52 cases) and group B after lower abdominal double cannula conduction (39 cases).TO compare ifferences of postoperative drainage volume, pelvic lymph cyst incidence , lower extremity venous thrombosis incidence and the incidence of urinary retention in two group.Re-sults The total volume of drainage after operation in A group (201.90 ± 42.60ml) were significantly higher than that of B group (127.62 ± 28.47ml), Pelvic lymph cyst, venous thrombosis incidence in A group were lower than those of B group (P<0.05);postoperative urinary retention rate in group A and group B were similar, the difference was not statistically significant.Conclusion For a row of pelvic lymph node dissection postoperative patients, us-ing transvaginal T tube drainage can reduce postoperative lymphoceles, venous thrombosis incidence, iti was ben-eficial for patients with postoperative recovery,and it was an effective drainage method and should be recom-mended widely.%　　目的：探讨盆腔淋巴清扫术放置T管经阴道引流对预后的影响。方法：选择2004年3月～2010年4月91例宫颈癌和子宫内膜癌行子宫根治术及双侧盆腔淋巴清扫术后患者：A组52例行经阴道T管引流，B组39例经下腹部双套管引流。比较两组术后引流量、盆腔淋巴囊肿发生率下肢静脉血栓发生率和尿潴留发生率的差异。结果：术后引流量A组（201.90±42.60mL）明显多于B组（127.62±28.47mL），盆腔淋巴囊肿、下肢静脉血栓发生率A组均低于B组(P<0.05)；术后尿潴留发生率A组与B组相似，差异无统计学意义(P＞0.05)。结论：对于行盆腔淋巴清扫术后患者，采用经阴道T管引流有利于降低术后淋巴囊肿、下肢静脉血栓发生率，利于患者术后恢复，是值得临床推广的一种有效引流方法。
  • 摘要：目的：分析急性股骨颈骨折患者血清中超敏C-反应蛋白(CRP)，神经元特异性烯醇化酶(NSE)及血清基质金属蛋白酶-9(MMP-9)的动态变化与疾病严重程度及预后的相关性。方法：选取2011年2月至2012年12月伤后急诊入院的股骨颈骨折患者60例为试验组。以同期门诊体检37例作为健康对照组。分别于发病后24小时以内，3天，7天抽取空腹外周、静脉血检测其CRP、NSE、MMP-9的血清浓度。结果：急性股骨颈骨折患者组患者均明显高于对照组患者，外周血清CRP水平24小时内明显高于受伤7天时和3天时；外周血清NSE水平24小时内和3天时均明显高于7天时；外周血清MMP-9水平在24小时内及3天时均明显高于7天时。结论：三项指标均在受伤早期即升高，半衰期短、下降迅速，因此CRP、NSE、MMP-9血清浓度变化对临床病情的判断，病情转归的预测。对指导治病具有重要的意义。%Objective Fracture analysis of serum high-sensitivity C-reactive protein in acute femoral neck ( CRP ), neuron specific enolase ( NSE ) and serum matrix metalloproteinase-9 ( MMP-9 ) the dynamic changes of correlation with disease severity and prognosis.Methods 60 patients from 2011 February to 2012 as the test group in December after injury emergency admissions of femoral neck fracture. To the same period 37 cases of outpa-tient medical examination as healthy control group. The difference in incidence after 24 hours, 3 days, 7 days of fasting peripheral, serum concentrations of CRP, NSE, MMP-9. Results Patient groups were significantly higher than the control group of patients with acute femoral neck fracture, the serum levels of CRP within 24 hours was significantly higher than that of the injured 7 days and 3 days;the serum levels of NSE in 24 hours and 3 days were significantly higher than that in 7 days;peripheral serum MMP-9 level in 24 hours and 3 days were signifi-cantly higher than that in 7 days and .Conclusion The three indexes in the injured early rise, short half-life, de-creased rapidly, so the CRP, NSE, MMP-9 serum concentration on clinical judgment, prediction of the outcome of illness. It is of great significance to cure.
  • 摘要：目的：探讨带神经血管蒂指背皮瓣在修复拇指损伤、皮肤软组织缺损的临床应用效果。方法：对本院2010年5月~2011年12月31例应用带神经血管蒂指背皮瓣修复拇指损伤、皮肤软组织缺损的临床资料进行回顾性总结分析。结果：31例皮瓣均成活，无静脉危象及明显肿胀发生，切口均Ⅰ期愈合。术后皮瓣色泽、质地与伤指皮肤相近，外形良好且指腹饱满，皮肤质地弹性好，耐磨，色泽稍淡，感觉恢复良好，静止两点辨别觉5~9 mm，捏、持动作恢复良好。结论：带神经血管蒂指背皮瓣修复拇指损伤、缺损手术操作简单，手术风险低，是修复拇指损伤，皮肤软组织缺损修复的可行方法。%Objective To analyze and discuss the clinical application of neurovascular pedicle fascial flap in repairing skin and soft tissue defect of thumb trauma. Methods The clinical datum of 31 cases from 2010 May~2011 December in our hospital applied neurovascular pedicle fascial flap in the repairing of skin and soft tissue defect of thumb trauma were analyze and discussed retrospectively. Results The cuts of 31 cases of skin and soft tissue defect of thumb trauma applied neurovascular pedicle fascial flap were healed. The color of flap skin and texture were close to normal. The injured finger was in good shape with elasticity, abrasion resistance. The sensory were recovered, the static two-point discrimination is 5~9 mm. Conclusion Surgical procedure of neurovascular pedicle fascial flap in the repairing of skin and soft tissue defect of thumb trauma is simple and safe, the risk is low and the effects were significant.
  • 摘要：Objective To prepare sinomenine hydrochloride ethosomes gel and to establish its quality con-trol metrod. Methods The sinomenine hydrochloride ethosomes were prepared by injection method and the opti-mal formation and preparation were selected by means of Orthogonal experiment. The ethosomes gel was pre-pared using mixing method.The content of sinomenine hydrochloride was determined by the HPLC and the en-trapment of the ethosomes was measured by dialytic method. Results The preparation obtained was canary gel and good in spreading. The shape of the sinomenine hydrochloride ethosomes was spherical with average encap-sulation efficiency of 62.8%.The linear range of sinomenine hydrochloride was 5~80μg·mL-1 (r=0.9999) .The average recovery of sinomenine hydrochloride was 98.5%. Conclusion The preparation method was simple and reasonable and its quality is stable and controllable.%　　目的：制备盐酸青藤碱醇质体凝胶并建立其质量控制方法。方法：以注入法制备醇质体，通过正交设计优选最佳处方和工艺；采用研和法制备醇质体凝胶；高效液相色谱法测定盐酸青藤碱的含量；透析法测定醇质体的包封率。结果：所得制剂为淡黄色透明凝胶，涂展性好；醇质体形态圆整，粒径小而均匀，平均包封率为62.8%。盐酸青藤碱检测浓度线性范围为5～80μg/mL (r=0.9999)，平均回收率为98.5%。结论：本制剂制备工艺简单可行，重现性好，质量稳定可控。
  • 摘要：目的：了解团体箱庭疗法、团体心理辅导对大学新生适应不良的干预效果，分析两者干预效果出现差异的原因。方法：采用《中国大学生适应量表》（CCSAS）和《症状自评量表》（SCL-90）筛选出14名适应不良的大学新生，将他们分成2组，分别采用限制性团体箱庭疗法和以团体活动为主的团体心理辅导进行干预研究。结果：团体箱庭疗法和团体心理辅导对缓解大学新生适应不良都具有良好的效果，但团体箱庭疗法在缓解情绪适应的效果要优于团体心理辅导。结论：团体箱庭疗法对大学新生适应不良干预效果的持续性更优于团体心理辅导。%Objective To find out the effects of group sandspiel and group psychological counseling for the New College Students Adapting the Bad Intervention Effect, analysis of the causes of the difference between the two intervention effect. Methods This study selected 14 non-adaptive freshmen throughChina College Student Adjustment Scale(CCSAS) and Symptom Check List-90(SCL-90), we divided them into 2 groups, sepa-rately adopted the restricted Group Sandspiel and the Group Psychology Counseling, which is mainly based on ac-tivity to do the intervention study. Results Both the Group Sandspiel and the Group Psychology Counseling have good defects on the new college students to relieve non-adaption. But the Group Sandspiel is superior to the Group Psychology Counseling on the effect of relieving emotional adaption. Conclusion The Group Sandspiel is even superior to the Group Psychology Counseling on the effect of adapting the bad intervention.
  • 摘要：目的：初步探讨臭氧应激建立气道高反应（AHR）大鼠模型的机制。方法：20只SD大鼠随机分为正常对照组和臭氧应激组，每组10只。通过呼气相气道阻力（Re），支气管肺泡灌洗液（BALF）总蛋白、细胞计数、分类，肺组织病理切片等指标确定AHR大鼠模型建立的可靠性；同时测定血浆IgE水平，外周血单个核细胞（PBMC）培养后取上清物测定IFN-γ、IL-13，对O3应激引发AHR的机理进行初步探讨。结果：臭氧应激组与正常对照组比较，气道反应性，BLAF中细胞计数、分类，血浆IgE水平等各项指标均升高；肺组织病理切片存在明显的炎性细胞浸润；PBMC培养上清中IFN-γ、IL-13臭氧应激组明显升高。结论：外源性损伤因子O3攻击支气管上皮细胞，气道上皮细胞功能缺陷或失稳态，引发气道高反应，其机制可能与Th1及Th2类免疫反应同时激活嗜酸性粒细胞（EOS）炎症反应有关。%Objective To investigate the mechanisms to establish airway hyperresponsiveness (AHR) rat model by ozone stress. Methods 20 SD rats were randomly divided into normal control group and ozone group (n=10). Indicators including resistance of expiratory airway (Re), total protein in bronchoalveolar lavage fluid (BALF), cell count、classification, lung tissue biopsy to confirm the reliability of AHR rat model;detecting the IgE level in the serum simultaneously:after peripheral blood mononujclear cells (PBMC) culture, adopting the super-natant to detect the IFN-γ,IL-13, the mechanism that caused the AHR was primarily discussed. Results Com-paring the ozone group with normal control group, all of the indicators, including airway responsiveness,cell count and classification in the BLAF and IgE level in the serum elevated; lung biopsy showed obvious inflammatory cell infiltration;For the ozone group, IFN-γ、IL-13 in supernatant of PBMC culture elevated evidently compared with the normal control group. Conclusion After xogenous damage factor O3 attacked the bronchial epithelial cells, the airway epithelial cells function defected and homeostasis lost, causing airway hyperresponsiveness, whose mechanism may be related to eosinophils (EOS) inflammation activated by Th1 and Th2 type immune re-sponses simultaneously.
  • 摘要：目的：评价非高危因素的腹腔镜下胆囊切除术（LC）患者预防应激性溃疡（SU）的必要性，为临床合理用药提供参考。方法：将90例非高危因素的腹腔镜下胆囊切除术患者按1：2的比例随机分为未用药组和用药组，并进行回顾性分析：术后SU的发生率；住院时间、住院费用、药品费用和原有疾病治愈率；不良反应发生率。其中未用药组30例，不进行SU的预防；用药组60例，使用抑酸药（ASD）预防SU。结果：未用药组与用药组均未出现术后SU和严重不良反应；二者在住院时间、住院费用、药品费用和原有疾病治愈率上的差异均无统计学意义；未用药组平均住院费用和平均药品费用均低于用药组。结论：对于不存在高危因素的LC患者，预防应激性溃疡是没有必要的。%Objective To evaluate the necessity of stress ulcer (SU) prophylaxis in laparoscopic cholecystec-tomy (LC) without risk factors. Methods A total of 90 patients were included and randomly divided into non-prophylaxis group (n=30) and prophylaxis group (n=60). Analysis indicators included SU rate;duration of hospi-talization, hospitalization cost, drug cost and the cure rate of disease; Results Both of two groups didn't appear SU and adverse reaction; There is no significant differences between two groups in duration of hospitalization, hospitalization cost, drug cost and the cure rate of disease;The non-prophylaxis group take a lower average hos-pitalization cost and drug cost than the prophylaxis group. Conclusion It is not necessary to take SU prophylaxis in Surgical Patients without Risk Factors.
  • 摘要：Objective To investigate the curative effect and safety of laparoscopic radical cystectomy. Methods We retrieved articles of randomized controlled trials, which are published after 1999 and related to the analysis between the effects and safety of LR and open resection of radical cystectomy. We then extract data and did Meta analysis. Results 7 articles were included in the Meta analysis. There is no difference on the general characteristics between the two groups.Compared with the OR group, the blood loss was less by 180.1ml, however, the operation time was 31.4min more than the other group. The volume of resected specimen was 18. 3cm3 in LR group rather than OR group. There were no significant differences in the number of retrieved lymph nodes,and the rates of urine leakage and incision infection is less in LR group. In addition, there were no significant differ-ences in the rates of hydronephrosis, urinary tract infection, electrolyte disorder, deep venous thrombosis, cancer recurrence and cancer metastases. Moreover, LR group has hospital stay time 4.5days less than OR group. The mortality rate 3 years in LR group was as same as that in OR group. Conclusions LR is as safe and effective as OR, in radical cystectomy,and do less injure to patients.%　　目的：评价腹腔镜膀胱癌手术的安全性和近期疗效。方法：检索1999年起到目前已发表的有关比较腹腔镜(LR)和开放手术(OR)行根治性膀胱切除术的临床疗效和安全性的前瞻性随机对照试验研究的有关文献，并根据其中数据进行Meta分析。结果：最后有7项RCT纳入分析，腹腔镜组与开放组患者一般情况无明显差异。腹腔镜组手术出血量较开放组减少180.1mL，但手术时间相对延长31.4min。切除肿块体积较开放手术减少18.3cm3。切缘情况和清扫淋巴结数量两组比较无差异不显著。腹腔镜组术后常见并发症如尿漏、肺部感染及切口感染均低于开放手术组；术后发生肾积水、泌尿系感染、电解质紊乱、深静脉血栓和肿瘤转移复发两组无统计学差异；平均住院时间比开放组少4.5天。术后3年生存率无明显差异。结论：腹腔镜下行膀胱癌根治性切除术是安全可靠的，可以达到根治切除的目的。
  • 摘要：Objective To explore the dynamic changes and value of the serum amyloid A protein, procalci-tonin and C-vreactive protein during the early process of acute pancreatitis. Methods 42 patients were divided into two groups:mild acute pancreatitis (n=32) and severe acute pancreatitis(n=10).All patients were employed for measuring the serum levels of SAA, PCT and CRP in patients on days 1, 3, 5, 7 of onset of acute pancreatitis, 42 health volunteers were enrolled as control group. Results (1) SAA, PCT and CRP levels in patients with acute pancreatitis were significantly higher than those in control group. (2) The levels of CRP of two groups reached the peak in day 3, and there was no difference in 3 days,But there was difference between MAP and SPA group after days 3. (3) the peak serum level of SAA in both SAP and MAP groups occurred on day 1, decreased with the treatment . (4) PCT level reached the peak in day 1 and day 3 for SAP group, day 1 for MAP group respectively, decreased with the treatment as well. Conclusion The changes of serum SAA, PCT and CRP levels manifest posi-tive correlations to the severity of the condition of the patients with acute pancreatitis, serum SAA and PCT level can be more informative than serum CRP level in evaluating the changes of the conditions in early acute pancre-atitis.%　　目的：观察急性胰腺炎患者血清淀粉样蛋白A(serum amyloid A protein，SAA)、降钙素原（procalci-tonin，PCT）和C反应蛋白（C-reactive protein，CRP）在急性胰腺炎早期病程中的变化，探讨SAA、PCT及CRP在急性胰腺炎其临床实用价值。方法：轻型急性胰腺炎（MAP）患者32例，重症胰腺炎(SAP)患者10例，动态检测入院时和入院后第1、3、5、7天血清SAA、PCT及CRP的水平，并均与42例健康对照组相互比较。结果：（1）急性胰腺炎患者各个不同时间点SAA、PCT及CRP均显著高于对照组。（2）其中CRP在入院后第3天达最高水平，随后逐步降低，第3天后两组差异显著；在MAP组于入院后第3天达峰值，随后显著下降并逐步恢复正常水平，而SPA组一直保持在较高的水平。（3）SAA于SAP组、MAP组均在入院后第1天为最高水平，随后逐步降低，各时间点与第1天比较，差异显著。（4）PCT在SAP组入院后第1天、3天为最高水平，在MAP组第1天为最高水平，随后均逐步降低，各时间点与第1天比较，差异显著。结论：血清SAA、PCT及CRP水平的变化反应了急性胰腺炎患者病情严重程度，其中SAA和PCT对急性胰腺炎早期病程判断具有一定的临床意义。
  • 摘要：目的：探讨坦索罗辛0.2mg联合非那雄胺5mg对老年前列腺增生症患者的焦虑及睡眠的影响。方法：观察35例老年前列腺增生症患者。年龄56-84岁,平均70岁。入选的35例BPH患者，口服坦索罗辛0.2mg加非那雄胺5mg，疗程三个月，观察患者服药前和服药后的国际前列腺症状评分表IPSS、焦虑程度SAS、阿森斯失眠量表AIS及生活质量评分表QOLS。结果：服药前有19例（54.3%）伴焦虑，服药后10例（28.57%）伴焦虑；服药前16例（45.71%）伴失眠，服药后8例（22.86%)伴失眠。服药前、服药后患者焦虑、睡眠障碍发生率比较，差异,有统计学意义。服药前后IPSS从25.00±3.93到15.85±3.51、SAS从38.06±6.10到26.50±5.72，AIS从10.60±5.06到5.00±4.22，QOLS从3.95±0.76到3.10±0.641，治疗前后比较有统计学意义。结论：坦索罗辛联合非那雄胺治疗前列腺患者能够缓解下尿路症状，改善老年前列腺增生症患者的焦虑、睡眠及生活质量。%Objective To evaluate the effect of tamsulosin (0.2mg) and finasteride (0.5mg) on anxiety and sleeping for benign prostate hypertrophy patients . Metheods Observed 35 benign prostate hypertrophy patients Patients were treated with tamsulosin 0.2mg,qd and combined with finasteride 0.2mg,qd for 3 months . IPSS,SAS, AIS and QOLS were measured before and after the treatment. Results Before taking the medicine, 19 cases (54.3%) with anxiety, after the treatment,10cases (28.57%) with anxiety ; Before taking the medicine16cases (45.71%) with insomnia , after the treatment, 16 cases (45.71%) with insomnia. There was significant difference between before the treatment and after the treatment. P<0.05 .Before the treatment ,average IPSS 25.00±3.93, av-erage SAS 38.06±6.10, average AIS 10.60±5.06, and average QOLS 3.95±0.76;After 3 months treatment , average IPSS 15.85±3.51. average SAS 26.50±5.72, average AIS 5.00±4.22 and average QOLS 3.10±0.641. There was significant difference between before the treatment and after the treatment. P<0.05. Conclusion Treatment of benign prostatic hyperplasia (BPH) with tamsulosin hydrochloride Sustained-Release Capsules combined with fi-nasteride Tablets could relieve lower urinary tract symptoms, improve anxiety, sleeping and the quality of life
  • 摘要：Objective To establish the control method for Olmesartan medoxomil and its related substances with RP-HPLC. Methods:The analysis was conducted on Inertsil WelchromC8 (4.6mm×150mm,5um) with a mo-bile phase of acetonitrile-phosphate buffer by gradient elution at a flow of 1.0ml/min. The column temperature was 25 and the detection wave length was 256nm.Result:The retention times of olmesartan medoxomil and its six relevant impurities are determined ;the separation is good and the purity of the related substances are less than 0.1%. Conclusion:This method is accurate,specific and can be used for the quality control of Olmesartan me-doxomil.%　　目的：建立RP-HPLC法测定奥美沙坦酯原料药含量及其有关物质的方法。方法：色谱柱为WelchromC8柱（4.6*150,5um），以乙腈-磷酸缓冲盐溶液为流动相进行梯度洗脱，流速为1.0ml/min，柱温25℃，检测波长256nm。结果：确定了奥美沙坦酯合成中引入的6个杂质以及奥美沙坦酯的峰位置，主峰能与相邻杂质峰较好的分离。杂质含量均小于0.1%。结论：采用反相高效液相色谱方法简准确、快捷，可有效的控制奥美沙坦酯中的有关物质。
  • 摘要：Objective The detection of acute coronary syndrome (ACS) patients with peripheral serum uric acid ( SUA ), homocysteine ( HCY ), adiponectin (ANP) levels and coronary artery lesion count. Methods Choose to live in Department of Cardiology of our hospital was diagnosed with CAG 170, ACS group ( n=97 ), SA group ( n=36 ) and group NCHD ( n=37 ). ELISA were detected in the peripheral serum SUA, HCY and ANP levels;coro-nary lesion vessels according to CAG evaluation. Results 1 patients in the ACS group, serum SUA, HCY and ANP levels in group NCHD and group SA were compared the level of statistical significance ( P<0.05 ). There was statistical significance in ACS patients peripheral serum levels of SUA, HCY and ANP levels and coronary artery lesion count difference ( P<0.05, P<0.01 ). Conclusion 1, ACS groups of patients with serum SUA, HCY and ANP levels that may be related to the pathophysiology of coronary vulnerable plaque. There was statistical significance in 2, ACS in peripheral serum SUA, HCY and ANP levels and coronary lesion vessels, further sug-gesting the level of their peripheral serum group with coronary atherosclerosis development.%　　目的：检测急性冠脉综合(ACS)征患者外周血清血尿酸(SUA)、同型半胱氨酸(HCY)、脂联素(ANP)水平及冠脉病变支数的关系。方法：选择住我院心内科经CAG诊断的患者共170人，其中ACS组(n=97)、SUA组(n=36)和NCHD组(n=37)。以ELISA分别测定患者外周血清SUA、HCY及ANP水平；冠脉病变支数根据CAG评定。结果：1、ACS组患者外周血清SUA、HCY及ANP水平与NCHD组和SA组患者水平比较有统计学意义。ACS患者外周血清SUA、HCY及ANP水平与冠脉病变支数比较差异有统计学意义。结论：l、ACS组患者外周血清SUA、HCY及ANP水平表明可能与冠脉易损斑块的病理生理相关。2、ACS患者外周血清SUA、HCY及ANP水平与冠脉病变支数有统计学意义，进一步提示它们外周血清水平的升高可能与冠状动脉粥样硬化发生发展相关。
  • 摘要：目的：探讨银杏叶提取物金纳多治疗脑梗塞患者的临床疗效以及对患者微循环的影响。方法：将90例脑梗塞患者随机分为治疗组和对照组。二组患者同时口服肠溶阿司匹林、尼莫地平、双嘧达莫进行常规治疗。治疗组另给予金钠多70mg加入生理盐水250ml静滴，每天一次，14天为一疗程。治疗结束分别检测90例脑梗塞患者微循环治疗前后差异及治疗组与对照组差异。结果：治疗前两组患者的各项指标差异不具有显著性，经应用金纳多后治疗组明显优于对照组。结论：金纳多能明显改善脑梗塞患者的微循环障碍。%Objective Discuss Ginaton treatment the curative effect of cerebral infarction patients and the influence of microcirculation on the patients. Methods 90 patients with cerebral infarction were random, divided into treatment group and control group.Patients in both groups takeorally enteric aspirin, Nimodipine , dipyri-damole to conventional therapy .Treatment group is given Ginaton 70 mg of sodium to join other physiological saline 250 ml static drops, once per day, every 14 days for a course of treatment. At the end of treatment , 90 cas-es of cerebral infarction patients was detected in microcirculation with the treatment group and control group dif-ferences before and after the treatment.Results each index of two groups of patients before treatment has no sig-nificant difference, the application of Ginaton treatment group is superior to the control group.And the difference was extremely significant (P< 0.01).Conclusion Ginaton can greatly improve the microcirculation handicapped patients with cerebral infarction.
  • 摘要：Objective To evaluate the efficacy of the combination of PureceptionTM gradient centrifugation techniques for sperm separation and ovarian stimulation in AIH. Methods The semen was prepared by Purecep-tionTM gradient centrifugation after the ovarian stimulation as a treatment, and the selected sperm suspension was used for AIH. Results After preparation, the density of sperm was increased from (47.13±22.97)×109/L to (69.32± 12.65)×109/L, the motility of sperm was raised from (42.27±12.33%) to (83.20±10.21%) as well, while the abnor-mal sperm was reduced from (62.42±23.12%) to (38.28±17.24%), the differences were statistical significant (P<0.01). The study includes 59 infertile couples who received 113 AIH cycles. The clinical pregnant rate per cycle was 9.10%in nature cycles (33 cycles), and 15.00%in ovarian stimulation cycles (80 cycles). Conclusion Pure-ceptionTM gradient centrifugation technique combined with ovarian stimulation in AIH, the clinical pregnant rate is satisfactory.%　　目的：探讨促排卵联合PureceptionTM 密度梯度法优选精子在AIH中的应用。方法：采用促排卵治疗后,用PureceptionTM 密度梯度离心法处理精液,并将优选后的精子应用于AIH治疗周期。结果：精液处理后,精子密度由处理前(47.13±22.97)×109/L提高到(69.32±12.65)×109/L，活率由42.27±12.33%提高到83.20±10.21%,畸形精子率由62.42±23.12%降至38.28±17.24%，差异有显著性。59对夫妇共进行了113个AIH治疗周期,自然周期（33例）AIH妊娠率9.10%,促排卵周期（80例）AIH妊娠率15.00%,差异有显著性。结论：PureceptionTM密度梯度法是一种较好的精子优选方法,联合促排卵治疗可以获得获得较满意的AIH临床妊娠率。
  • 摘要：目的：探讨肾上腺意外瘤中醛固酮瘤的左心室肥厚情况。方法：肾上腺意外瘤中醛固酮瘤患者21例(A组)和有症状的醛固酮瘤患者30例(B组)，分别记录一般临床资料和生化指标，并进行超声心动图检查。对两组患者的临床特征、生化检测结果和左心室肥厚程度进行比较分析。结果：(1) B组左室后壁厚度、LVM、LVMI高于A 组。(2)醛固酮瘤患者LVMI与立位血浆醛固酮独立相关。(3)B组左室肥厚发生率高于A组。结论：肾上腺意外瘤中醛固酮瘤的醛固酮分泌轻度升高可导致左心室肥厚。%Objective To investigate the left ventricular hypertrophy in patients with aldosteronoma in a-drenal incidentaloma.Methods 21 patients with aldosteronoma in adrenal incidentaloma (group A) were evaluated, all of whom were recorded clinical data, biochemical indicators, and examined by echocardiography. These data were compared with those of 30 typical aldosteronoma (group B). Results ⑴Group B patients had significant greater left ventricular posterior wall thickness (PWd), LVM, LVMI compared with group A patients. ⑵Multiple regression analysis showed a correlation between LVMI and upright plasma aldosterone in aldosteronoma(P<0.05). 3)the incidence of left ventricular hypertrophy in group 2 was higher than in group 1 (P<0.05). Conclusion aldos-teronoma in adrenal incidentaloma, whose aldosterone secretion mild elevated could lead to left ventricular hyper-trophy.
  • 摘要：Objective The goal of this study is through dose studies provide theoretical basis for SBRT used in central lung cancer;to compare the dosimetric distribution of tumor target and surrounding normal tissue be-tween different radiothe-rapy plannings of the non-small-cell lung cancer,Methods The plannings conclued 9-beam DMLC-IMRT,3DCRT,7- beam DMLC-IMRT.Compared the three plannings in the difference of the dosi-metric distribution for the target and normal tissue. Results SBRT can be used in central lung cancer;7beam IM-RT plannings are better in protect the normal tissues. Conclusion SBRT can be used in central lung cancer;Compared to other two plannings ,7 beam IMRT plannings have advatages in clinical,is worth to promotion fur-ther.%　　目的：通过剂量学研究为立体定向放疗（SBRT）应用于中央型肺癌提供理论依据；通过比较中央型肺癌在三维适形放疗（3-DCRT）、动态调强放疗（DMLC-IMRT）技术下实现SBRT后肿瘤靶区和周围正常组织的剂量学差异，为SBRT应用于中央型肺癌提供剂量学依据。方法：对近1年内收治的30例中央型非小细胞肺癌患者设计处方剂量和治疗间隔相同的9野IMRT、3-DCRT和7野IMRT立体定向放疗（SBRT）计划，采用剂量体积直方图评价不同计划靶区和正常组织照射剂量以及适形指数（CI）和不均匀性指数（HI）。结果：SBRT应用于中央型肺癌的满足剂量学要求；三组计划比较，7野IMRT较其他两种计划对正常组织的保护性更好。结论：从剂量学角度分析SBRT可以应用于中央型肺癌；7野IMRT计划较其他两种计划在剂量学上更有优势。
  • 摘要：Objective Aim To investigate the effects of 8-bromo-7-methoxychrysin (BrMC) on apoptosis of human ovarian cancer cells and its phosphorylated Akt(p-Akt) expression during the process of apoptosis. Meth-ods Human ovarian cancer A2780/DDP cells cultured in vitro were treated with various concentration of BrMC. The apoptotic cell death was examined using the cell apoptosis ELISA detection kit and flow cytometry (FCM) af-ter propidium iodide (PI) staining. The level of p-Akt protein expression was analyzed by Western Blot. Results BrMC significantly induced apoptosis of A2780/DDP cells and down-regulated p-Akt expression level in a con-centration dependent manner. Conclusion BrMC induces apoptosis of human ovarian cancer A2780/DDP cells, and its mechanisms are involved in inhibition of Akt activaties.%　　目的：研究8-溴-7-甲氧基白杨素(8-bromo-7-methoxychrysin, BrMC)诱导人顺铂耐药卵巢癌A2780/DDP细胞凋亡作用及细胞凋亡过程中Akt蛋白磷酸化水平的变化。方法：用不同浓度的BrMC处理体外培养的A2780/DDP细胞，碘化丙啶(PI)染色流式细胞术(FCM)和细胞凋亡ELISA检测试剂盒检测凋亡性细胞死亡，Western Blot分析Akt蛋白磷酸化水平。结果：BrMC具有诱导A2780/DDP细胞凋亡作用，并且随药物浓度的增加而增强；同时BrMC以浓度依赖的方式下降Akt蛋白磷酸化水平。结论：BrMC诱导A2780/DDP细胞凋亡与其抑制Akt活化相关。
  • 摘要：Objective To investigate the clinical prognostic value of major abnormal cardiacevents (MACE) in unobese patients of acute coronary syndrome (ACS) patients before percutaneous coronary intervention (PCI )by plasma N-terminal pro-B-type natriuretic peptide(NT-proBNP).Methods A total of 101 unobese patients of acute coronary syndrome,which were divided into ≤80pg/ml group of 31 patients and 〉80pg/ml group of 70 patients . follow-up major abnormal cardiac events (including death,cardiogenic shock, recurrent angina pectoris, non-fatal re-infarction, non-fatal heart failure,arrhythmia,target vessel revascularization, re-admitted to hospital composite end point) within 6 months after PCI.Results 1.In un-obese patients,MACE、death and CHF times of patients with NT-proBNP>80pg /ml were significantly higher than these times of patients with NT-proBNP≤ 80 pg/ml (P<0.05);2.in patients unobese,NT-proBNP levels predicted MACE、death and CHFwas significant (P<0.001),Con-clusion This clinical study results show that NT-proBNP can predicted MACE、death and CHF in unobese pa-tients within 6 moths after PCI.%　　目的：探讨N 端脑钠肽前体（NT-proBNP）对非肥胖急性冠状动脉综合征（ACS）经皮冠脉介入（PCI）术后主要不良心脏事件（MACE）的预测价值。方法：因ACS 行PCI手术的非肥胖患者101例，分为低NT-proBNP水平组（NT-proBNP≤80 pg/ml）31例，高NT-proBNP水平（NT-proBNP＞80 pg/ml）组70例，统计并分析患者PCI术后6个月内MACE发生率。结果：高NT-proBNP水平组PCI术后MACE发生率高于低NT-proBNP水平组；NT-proBNP可独立预测非肥胖ACS患者PCI术后MACE发生率、死亡率、和心衰的发生率。结论：血浆NT-proBNP水平可用于评估非肥胖ACS患者冠脉病变的范围PCI术后短期（6个月内）的预后。
  • 摘要：Objective To study the laparoscopic technique in the diagnosis and treatment of surgical abdom-inal emergency application value and method Methods Retrospective analysis of 46 cases of application of la-paroscopy diagnosis and treatment of acute abdominal pain in patients with clinical data Results 43 cases (93.4%) completed in laparoscopic diagnosis and surgical treatment, 3 cases (6.4%), operation treatment, The con-trol group were significantly shorter than the control group, bleeding was significantly less than that in the control group, but the intraoperative diagnosis rate was significantly higher than that of the control group Conclusion Laparoscopy can be widely used in the diagnosis and treatment of patients with acute abdominal pain, but also need to pay attention to the application conditions.%　　目的：探讨腹腔镜技术在外科急腹症诊断和治疗中的应用价值和方法方法：回顾性分析研究我院46例应用腹腔镜技术诊疗的急腹症患者的临床资料结果：43例（93.4%）在腹腔镜下完成诊断和手术治疗，3例（6.4%）中转行开腹治疗，对照组住院时间明显短于对照组，术中出血量明显少于对照组，但术中确诊率却明显高于对照组。结论：腹腔镜技术可广泛应用于急腹症患者的诊断与治疗，但也需注意应用条件。
  • 摘要：目的：了解HIE患儿血清细胞因子水平的变化及其临床意义，并探讨其在HIE监测中的意义。方法：以2007年1月～2011年12月我院符合HIE诊断标准的新生儿为病例，随机抽取同期正常分娩的新生儿作为对照，用ELISA 双抗体夹心法检测2组新生儿出生后24h内（急性期）和出生后第7天（恢复期）的血清IL-1β、IL-6、IL-8、IL-10和TNF-α水平。结果：①急性期，HIE组患儿血清IL-1β、IL-6、IL-8、IL-10和TNF-α水平均高于正常新生儿，且随着HIE病情的加重，IL-1β、IL-6、IL-8、IL-10、TNF-α5种细胞因子水平均有显著性上升；②恢复期，HIE轻度患儿和正常新生儿的IL-6、IL-8、IL-10、TNF-α水平无显著性差异，HIE中度患儿和正常新生儿的IL-8、IL-10无显著性差异，HIE轻度患儿和中度患儿IL-1β、IL-8、IL-10无显著性差异，HIE中度患儿和重度患儿TNF-α无显著性差异；③急性期IL-1β、IL-6、IL-8、IL-10和TNF-α5种血清细胞因子水平都显著高于恢复期。结论：血清IL-1β、IL-6、IL-8、IL-10和TNF-α参与了新生儿HIE脑损伤过程，随着HIE病情的加重，血清细胞因子水平显著上升，在HIE脑损伤好转的过程中，细胞因子水平逐渐下降，其动态变化可作为HIE病程监测的重要指标。%Objective To find out the clinic significance of serum cytokines in newborn with hypoxie is-chemic ncephalopathy. Methods Collect 98 cases of HIE and 100 normal control in Chang Sha Central Hospital during the time from January 2007 to December 2011, use ELISA double antibody sandwich method to detect the serum IL-1β、IL-6、IL-8、IL-10 and TNF-αlevels within 24 hours (acute phase) and 7 days after birth(Conva-lescence). Results In acute phase, serum IL-1β, IL-6, IL-8, IL-10 and TNF-αlevels in HIE babies were high-er than those in normal babies, and with the HIE exacerbations, Serum Cytokines levels increased obviously; In Convalescence, IL-1β、IL-6、IL-8、IL-10 and TNF-αlevels were significantly lower than those in acute Period , and most serum Cytokines levels decreased to normal level. Conclusion The serum IL-1 β, IL-6, IL-8, IL-10 and TNF-αparticipate in HIE of newbeorn, with the HIE exacerbations, serum cytokines levels significantly in-creased, and with the HIE brain injury improves, serum cytokine levels gradually decreased, the serum cytokines changes can be used as an important index in monitoring the course of HIE.
  • 摘要：Objective To compare the effeets of two types of surgery on treatment of primary angle-closure glaucoma with cataract. Methods The indications of two types of microsurgery were set up for non-randomized comparison study on 30eyes ( 27cases ) of primary angle-closure glaucoma with cataract patients, who were per-formed phacoemulsification with IOL (Phaco+IOL) and phacortrabeculectomy plus intraocular lens implantation (Phaco Trab+IOL group). The preliminary clinical results of two groups were compared. The follow up visit time was 8~15 months. Results The postoperative IOP in two groups were well controlled postoperatively compared with preoperatively (both P<0.05). The chamber angle was widened and the visual field was increased in most pa-tients of the two groups. No severe intraoperative complications happened in two groups. Conclusion The indica-tions of two types of microsurgery will be benefits for treatment on primary angle-closure glaucoma.with cataract. Phacoemulsification combined with artificial lens implantation for angle-closure glaucoma is safe and effective, which can improve visual acuity and decrease intraocular pressure without inducing severe complications intra and post operation. The glaucoma cataract combined surgery demand the patients have a good condition in the in-traocular pressure and the perfect suitable surgery opportunity, and more the surgery is more complex and hard, but it has good clinical therapeutic effects&prognosis also. The two types of microsurgery must be adopted to in-dications.%　　目的：探讨治疗原发性闭角型青光眼伴白内障两种手术方式的适应症、临床疗效及预后。方法：采用非随机临床对照研究方法，拟定手术适应症，对原发性闭角型青光眼伴白内障30眼（27例），进行手术治疗，采用单纯超声乳化白内障吸除+晶体囊内折叠人工晶体植入术（Phaco+IOL）和青-白联合手术（复式小梁切除联合超声乳化白内障吸除+晶体囊内折叠人工晶体植入术（PhacoTrab+IOL），比较不同适应症下两种手术方式初步的临床疗效，随访8~15个月。结果：两组术后随访期内均能将眼压控制在理想范围，术后眼压较术前明显降低，P值<0.05；大部分患者房角较术前增宽，视野增大，前房深度明显加深，二组均未见严重术中并发症。结论：不同手术方式适合不同的病人情况，Phaco+IOL是一种可选用的安全有效的手术方法，能有效提高视力与降低眼压，无术中和术后严重并发症发生；PhacoTrab+IOL则需要患者的眼压情况控制良好，掌握手术时机，操作相对复杂，难度相对较大，但也能取得较好的临床疗效和预后。两种术式的选用要注意适应症的选择。
  • 摘要：目的：了解在渝三级甲等医院医务人对健康教育专业知识的掌握及其在具体工作中的实际应用情况，探索影响健康教育开展的因素。方法：采用先整群后随机的多级抽样方法，用自编问卷对337名医务人员进行调查分析。结果：337名医务人员对健康教育专业知识平均知晓率为76.1%；医务人员中62.6%的人认为健康教育有很大作用，89.6%的人愿意接受专门培训，45.4%的人表示在医护工作中经常进行病患健康教育，49.6%的人经常干预病人不良行为。结论：不同学历、职业、职称的医务人员对大部分健康教育专业知识的知晓率无显著差异；他们对健康教育态度较积极，但实际运用不够，仍有必要经常对医务人员进行健康教育专业知识培训。%Objective To understand the mastery and application on professional knowledge of health edu-cation of the medical staff in some Grade III Level A hospitals in Chongqing, and research the factors about the health education of hospital. Methods The medical staff of 337 was sampled by multistage sampling method con-sisted of cluster sampling and random sampling, and they were inquired by a health education questionnaire was made by us. Results To the 337 cases, the average awareness of health education knowledge was 76.1%, about 62.6%of the medical staff thought that health education was very important, 89.6%of them was willing to receive health education training, 45.4%of them often practiced health education to patients, and 49.6%of them often in-tervened the patients' un-health behavior. Conclusion To the mastery on health education of the medical staff, there were no significant differences among different education background, different profession and different ti-tle. Their attitude was positive for health education, but they practice inadequately in their actual work. It is nec-essary to improve the training rate of health education for the medical staff and to raise their professional qualities and skills.
  • 摘要：Objective Study the correlation between the activities of men and women's hair follicle cells , white hair and black hair and the related Ag-NORS in the nuclear organizer region by means of male and fe-male's hair follicle cell proliferation activity determination of nucleolus organizer regions of silver autophagy pro-tein determination. Methods Collect the hair of fifty people of all ages and both sexes and extract the hair folli-cle cells from their hair, then put the nucleolus which is silver producer under the microscope to count the num-ber of hair follicle cells of one thousand and one hundred people of all ages and both sexes and the number of Ag-NOR. Results Young men's activities of hair follicle cells are no different than those of young women, while there is a big difference between the activities of hair follicle cells of the old people's white hair and those of young people. Conclusion Our experiment demonstrates that the number of AgNOR particles with high estimation value of cell activity.%　　目的：通过对男女性头发毛囊细胞增殖活性的核仁组成区嗜银蛋白（Ag-NORs）测定，定量分析男女头发、白发与黑发性的活性与核仁组成区相关嗜银蛋白（Ag-NORs)的关系。方法：分别收集50名男女老少性的头发，提取毛囊细胞，用核仁银染制片，显微镜下分别计数1100个男女老少及白发毛囊细胞核并统计Ag-NOR的数目。结果：年轻男女毛囊细胞活性相差不大，而老人白发与年轻人的头发毛囊细胞活性相差较大。结论：AgNOR颗粒数目对细胞活性具有较高估测价值。

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