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首页> 中文期刊> 《湖南师范大学学报（医学版）》 >循环microRNA-122a和microRNA-6086在肝癌诊断及预后中的作用

循环microRNA-122a和microRNA-6086在肝癌诊断及预后中的作用

         
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摘要

目的:检测肝癌患者血清中miR-122a、miR-6086含量,研究其在肝癌诊断及预后中的作用.方法:采用real-time PCR方法进一步检测血清中miR-122a和miR-6086的表达水平.结果:观察组血清中miRNA-122a的相对含量低于对照组;miR-6086的含量高于对照组,差异均有统计学意义;统计分析发现,在不同年龄、分期、分型、肿瘤大小及AFP水平、肝功能等,患者间相对表达量差异均无统计学意义;MiRNA-122a的AUC面积为0.842,大于miRNA-6086的0.782 AUC面积,且对原发性肝癌的诊断的敏感性高于miRNA-6086,特异度无明显区别;MiRNA-6086的表达量越高患者的生存时间越短,相反表达量越低生存时间越长;miRNA-122a的表达量较正常人下调,但是其生存曲线上低表达量生存时间长于高表达的生存时间.结论:MiRNA-6086、miRNA-122a的表达水平较正常人有显著差异,可以作为肝癌诊断及预后的新指标.%Objective The purpose of this study is to investigate the expression of miRNA-6086,miRNA-122a in the se-rum of normal and primary hepatocellular carcinoma, and to research the relationship between the diagnosis and prognosis of hepatocellular carcinoma. Methods The miR-122a and miR-6086 in serum were detected by real-time PCR method. Re-sults The relative content of miRNA-122a in the experimental group was lower than that of the control group, but the content of miR-6086 was higher than that of the control group and the difference both were statistically significant. Statistical analysis revealed that there were no significant differences in the relative expression of the patients in different age, stage, classifica-tion, tumor size, AFP level, liver function and so on. AUC MiRNA-122a area was 0.842, greater than the 0.782 AUC area of miRNA-6086, which the sensitivity of the diagnosis of HCC was higher than miRNA-6086 and no significant difference in specificity. The higher expression level of miRNA-6086, HCC patients have shorter of survival time;the lower of expression level, HCC patients have longer of survival time. Although the expression level of miRNA-122a was down regulated, the lower expression of patients have longer survival time than the higher expression survival time. Conclusion MiRNA-122a and miR-NA-6086 expression levels in the serum of HCC were significantly different from those of normal persons, which could be used as potential targets for the diagnosis and prognosis of HCC.

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  • 来源
    《湖南师范大学学报（医学版）》 |2017年第2期|12-16|共5页
  • 作者

    张楚红; 文美玲; 李金成; 左建宏; 胡甜; 阳学风; 李赛; 吕秀; 贺莎; 严汉兴; 谭贻兮; 陈荣如;

  • 作者单位

    南华大学附属南华医院,衡阳421000;

    南华大学医学院,衡阳421000;

    南华大学附属南华医院,衡阳421000;

    邵阳学院医学部,邵阳422000;

    南华大学附属南华医院,衡阳421000;

    南华大学医学院,衡阳421000;

    南华大学附属南华医院,衡阳421000;

    南华大学医学院,衡阳421000;

    南华大学附属南华医院,衡阳421000;

    南华大学附属南华医院,衡阳421000;

    南华大学附属南华医院,衡阳421000;

    南华大学附属南华医院,衡阳421000;

    南华大学附属南华医院,衡阳421000;

    南华大学附属南华医院,衡阳421000;

    南华大学附属南华医院,衡阳421000;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 肝肿瘤;
  • 关键词

    肝癌; MiR-122a; MiR-6086;

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