Real-time quantitative PCR is widely used in the analysis of gene expression difference and etc ., due to its sensitivity , accu-racy and operability .It' s necessary to select the optimal reference gene for data analysis and the credibility of the experiment before qRT-PCR.In this study, β-actin, PDA1, ALG9, TDH3, TAF10 were detected by real-time quantitative PCR in Saccharomyces cerevisiae BY4741 and H4K5R under nickel stress, and the M values of 5 candidate referece genes were as follows:β-actin(0.808) 展开▼
