The interactions between lysozyme and hydroxy-functionalized ionic liquids including 1 - ( 2, 3 -dihydroxypropyl) -3 -methylimidazolium chloride ( [ 2, 3 -dhpmim ]C1), l-(2, 3 -dihydroxy-propyl) -3 -methylimidazolium tetrafluoroborate ( [ 2, 3 -dhpmim ] BF4 ) and 1 - ( 2, 3 -dihydroxypropyl) -3-methylimidazolium hexafluorophosphate( [2, 3-dhpmim ]PF6) were studied by UV -Vis absorption and fluorescence spectra. The results indicated that the fluorescence quenching of lysozyme by hydroxy-functionalized ionic liquids performed in static quenching mode. The synchronous fluorescence spectra showed that the microenvironment of tryptophan residues was changed. The binding constants and binding sites of ionic liquids to lysozyme increased by the order of [2, 3-dhpmim] Cl ＜ [2, 3-dhpmim]BF4 ＜ [2, 3-dhpmim]PF6, and raised with the increase of temperature.%采用荧光光谱和紫外吸收光谱研究了羟基功能化离子液体1-(1,2-二羟基丙基)-3-甲基咪唑氯盐([2,3 -dhpmim] Cl)、1-(1,2-二羟基丙基)-3-甲基咪唑四氟硼酸盐([2,3-dhpmim] BF4)、1-(1,2-二羟基丙基)-3-甲基咪唑六氟磷酸盐([2,3-dhpmim] PF6)与溶菌酶的相互作用.研究发现此3种离子液体对溶菌酶的荧光猝灭均为静态猝灭；同步荧光显示离子液体与溶菌酶肽链上的色氨酸残基作用,色氨酸残基微环境发生改变；结合常数和结合位点数按照[2,3-dhpmim] Cl、[2,3-dhpmim] BF4、[2,3-dhpmim] PF6顺序依次增大,并随温度的升高而增大.
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