Investigation on the adsorption of proteins on graphene is very important for the biomedical applications and safety evaluation of graphene materials. In this work, the adsorption of cytochrome c, a protein involved in cell apoptosis, on graphene oxide was studied. The morphology of graphene oxide and its protein complex were characterized by atomic force microscopy, indicating that the adsorption occured in multilayer mode. The adsorption kinetics and isotherm were also studied, revealing a very high adsorption capacity(1 978 mg/g) of cytochrome c on graphene oxide. Finally, it is found that the enzymatic activity of cytochrome c only changed slightly before and after the adsorption on graphene oxide, suggesting that the conformation of cytochrome c was maintained during the adsorption process. This work is expected to provide some important data for biomedical applications and safety evaluation of graphene materials.%蛋白质在石墨烯上的吸附研究对于石墨烯材料的生物应用及安全性评价具有重要意义.该文对一种具有重要生理意义的蛋白质——细胞色素c在氧化石墨烯上的吸附进行了研究,利用原子力显微镜对吸附前后的形貌进行了表征,考察了吸附动力学和最大吸附容量,并对蛋白质在吸附前后的酶活性进行了比较.结果表明,细胞色素c在氧化石墨烯上具有超高的吸附容量(1978 mg/g),吸附模式为多层吸附,且吸附作用力较牢固.吸附后,细胞色素c的酶活性仅有少许下降,说明蛋白质构象未发生变化.该文有望为石墨烯材料的生物应用和安全性评价提供重要数据.
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