应用单滴微萃取(SDME)-反相液相色谱(RPLC)检测了环境水样中的百菌清残留.优化了单滴微萃取条件:环己烷萃取剂6 μL、单滴体积2 μL、搅拌速率350 r/min、萃取时间40 min、水溶液温度35 ℃、无盐度.水样经单滴微萃取后,使用Hypersil C18柱反相液相色谱分离测定百菌清.反相液相色谱条件:100%甲醇流动相、流速1.0 mL/min、柱温25 ℃、224 nm检测.方法的线性范围、检出限、相对标准偏差和富集倍数分别为1.0 ～50 μg/L、0.02 μg/L、6.1%和427倍.采用该法对环境水样中的百菌清残留进行了测定,环境水样的加标回收率为98% ～106%.%A detection method for determination of chlorothalonil residue in environmental water samples by single drop microextraction(SDME)-reverse phase liquid chromatography(RPLC) has been studied.The conditions of effect on SDME have been optimized,and the optimal conditions were as follows:6 μL cyclohexane for extraction solvent,2 μL single drop,350 r/min for stirring speed,40 min for extraction,35 ℃ and 0% of salinity for water solution.After extracted by SDME,the sample was separated and determined by RPLC with Hypersil C18 column and 100% of methanol as the mobile phase with the flow rate of 1.0 mL/min at 25 ℃.The detection wavelength was set at 224 nm.The linearity,detection limit,RSD and enrichment fold of the method were 1.0-50 μg/L,0.02 μg/L,6.1% and 427,respectively.The proposed method has been applied to determine chlorothalonil residue in environmental water samples,and the recoveries from spiked environmental water samples varied from 98% to 106%.
展开▼
