In this study ,with the leaves of Perilla frutescen were used as explant ,effect of different concentrations of the hormone on inducing callus and formating flavonoid in Perilla frutescen leaves were studied by single factor and orthogonal test. It was found that the effect order of four kind of hormones to callus induction was 2,4-D>6-BA>NAA>KT and the order to flavonoid formation of the optimum medium for callus induction was MS+6-BA 2. 0 mg/L+2 ,4-D 0. 5 mg/L+KT 0. 6 mg/L+NAA 0. 8 mg/L by orthogonal design ,under the optimum conditions ,the highest indution rate was 83. 3%after 25 days. Furthemore ,the flavonoid formation of callus achieved at 3. 925%,it is much higher than the natural Perilla frutescen leaves ,the experimental results indicate the feasibility of flavonoid formation with tissue engineering.%以紫苏叶为外植体，采用单因素和正交设计试验研究了植物激素对紫苏叶愈伤组织诱导和黄酮积累的影响。结果表明：4种激素对紫苏叶愈伤组织诱导的影响依次为2，4-D>6-BA>NAA>KT；对愈伤组织积累黄酮的影响依次为2.4-D>6-BA>KT>NAA；紫苏叶愈伤组织培养的最佳激素组合为6-BA 2.0 mg/L+2，4-D 0.5 mg/L+KT 0.6 mg/L+NAA 0.8 mg/L；经愈伤组织培养获得的黄酮含量最高达到3.925%，远高于自然界紫苏叶黄酮含量。上述试验结果说明利用组培技术获得紫苏黄酮有可行性。
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