Six endophytic bacterial strains were separated from the Pinus massoniana twigs. The bioassay result showed that the mortality of Bursaphelenchus xylophilus treated for 24 h with Z11-2 strain and G36 strain were 92％ and 89％ respectively, with the decomposition rate 87. 4％ and 77. 5％ respectively for 48 h's treatment. By combination of the morphologic observation with 16S rDNA sequence analysis,according to the position in phylogenetic tree,we found that Z11-2 strain and G36 strain were identified as Bacillus pumilus,as showing 100％ and 99％ homology respectively.%为筛选出对松材线虫具有抑制作用的生物防治细菌,对马尾松内生细菌进行分离纯化,从中筛选出6株对松材线虫具有杀线虫活性的细菌,其中细菌Z11-2和细菌G36具有较强的杀线活性,用它们的培养滤液分别处理松材线虫24 h,松材线虫的死亡率分别达92％和89％,48 h后线虫消解率分别是87.4％和77.5％.通过细菌形态特征观察和16S rDNA测序及其系统发育分析,菌株Z11-2和G36与短小芽孢杆菌Bacillus pumilus处于同一分支,同源性分别为100％和99％,初步鉴定细菌Z11-2和G36为短小芽孢杆菌.
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