根据H7亚型禽流感病毒的血凝素基因中的保守区域设计特异的LAMP引物。10倍倍比稀释试验表明了此方法的高灵敏性，最低可检测到100 fg的RNA。对H1、H3、H4、H5、H6、H9亚型A型流感病毒和NDV进行H7－RT－LAMP检测，并无交叉反应，说明了此方法的高特异性。利用80份临床样品对所建立的H7－RT－LAMP方法进行评价，鉴定结果和病毒分离方法一致，说明此方法的可行性。建立的H7－RT－LAMP方法简单、快速、灵敏、特异，是H7亚型禽流感病毒的一种高效检测方法，适合在基层进行H7亚型禽流感病毒的早期筛检。%The RT-LAMP primers were designed to target the HA gene of H7 subtype of avian influ⁃enza viruses. Results of 10⁃fold dilution series assays showed that analysis of RT-LAMP products by the real⁃time fluorescence method was highly sensitive, and that the analytic sensitivities of this method was 100 fg. Using a pool of RNAs extracted from influenza viruses corresponding to H1, H3, H4, H5, H6 and H9 HA subtypes and NDV, the RT-LAMP system was confirmed to amplify only H7 AIV RNA. The performance characteristics of the RT-LAMP assay were evaluated with 80 clini⁃cal specimens collected from suspected poultry and wild birds, the results of which were consistent with virus isolation and demonstrated the feasibility of the method. Overall, the RT-LAMP assay es⁃tablished in this study is simple, rapid, sensitive and specific, which can be used as a reliable method for early diagnosis of the influenza A( H7N9) virus infection, especially in underequipped laboratories.
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