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首页> 外文会议>International Conference in One Health >Subtype Identification Of Avian Influenza Virus Isolated from Laying Duck In Sidenreng Rappang, South Sulawesi With Hemagglutination Inhibition Assay
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Subtype Identification Of Avian Influenza Virus Isolated from Laying Duck In Sidenreng Rappang, South Sulawesi With Hemagglutination Inhibition Assay

机译：南苏拉威病南苏拉威病株中孤立的禽流感病毒亚禽流感病毒的亚型鉴定，血凝集抑制作用

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Avian Influenza virus (AIV) have caused significant economic losses in poultry sector worldwide. Results of genetic mapping showed that circulating AIV in Indonesia were belong to clade 2.1.1, 2.1.2, 2.1.3 and 2.3.2, however the AIV subtype were merely H5N1. Our study aim to identify the hemagglutinin protein of AIV isolated from native laying duck in Sidenreng Rappang, South Sulawesi, Indonesia using HI assay. Three cloacal swab samples:Sidrap3 (S3), Sidrap5 (S5), and Sidrap10 (S10) were isolated from native laying duck at three duck farms at Sidenreng Rappang during AI outbreaks in 2016. These samples were propagated on spesific antibody negative (SAN) embryonated chicken eggs, followed by subtype identification using hemagglutination inhibition (HI) test using specific antibody of AIV subtype H5N1, AIV subtype H5N2, AIV subtype H5N3 and ND Lasota virus. Our study showed that H5N1 specific antibody has titer of 2~4 against virus isolates Sidrap3 and Sidrap10, titer of 2~6 against virus isolates Sidrap5. H5N2 specific antibody has titer 2~4 against virus isolates Sidrap3 and Sidrap5, titers of 2~6 against virus isolates Sidrap10. H5N3 specific antibody has titers of 2~6 against virus isolates Sidrap3, Sidrap5 and Sidrap10. Whilst, specific antibody of ND Lasota did not react with all of virus isolates. Our results showed that all AIV isolates gives highest reaction with specific antibody of AIV H5N3. According to the result, it is likely that AIV H5N3 might circulating among laying duck in Sidenreng Rappang.
机译：禽流感病毒（AIV）造成了全球家禽部门的重大经济损失。遗传映射的结果表明，印度尼西亚的循环AIV属于Clade 2.1.1,2.1.2,2.1.3和2.3.2，但AIV亚型仅仅是H5N1。我们的研究旨在识别在印度尼西亚南苏拉威西州南苏拉韦斯南苏拉韦岛的天然鸭肉中孤立的AIV的血凝素蛋白。在2016年的AI爆发期间，从Iidenreng Rappang的三只鸭子农场分离出三个核酸拭子样品：SiDRAP3（S3），SIDRAP5（S5）和SIDRAP10（S10）。这些样品在Spesific抗体负（SAN）上繁殖使用AIV亚型H5N1，AIV亚型H5N2，AIV亚型H5N3和ND Lasota病毒的特异性抗体，使用血凝抑制（HI）试验，使用血凝抑制（HI）试验进行胚胎鸡蛋。我们的研究表明，H5N1特异性抗体对病毒的滴度为2〜4，SIDRAP3和SIDRAP10，滴度为2〜6次对抗病毒分离株SIDRAP5。 H5N2特异性抗体对病毒的滴度2〜4分离物分离物SidraP3和Sidrap5，滴度为2〜6针对病毒分离物SIDRAP10。 H5N3特异性抗体具有2〜6的滴度，对病毒分离物Sidrap3，Sidrap5和Sidrap10。虽然，Nd vacota的特异性抗体与所有病毒分离株没有反应。我们的研究结果表明，所有AIV分离物都具有与AIV H5N3的特异性抗体的最高反应。根据结果​​，AIV H5N3可能会在侧支拉普康中铺设鸭子。

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