目的:检测卵巢功能调控相关蛋白在不同发育阶段卵泡中的表达,探讨其在卵泡发育及卵巢功能调控中的作用及其意义.方法:选取包含原始卵泡、初级卵泡、次级卵泡、成熟卵泡、闭锁卵泡及白体的育龄期女性卵巢组织,采用免疫组织化学法分析乙二醛酶I(Glyoxalase I)、泛素羧基末端水解酶L1(UCH-L1)、热休克蛋白27(HSP27)和血清淀粉样蛋白P(SAP)在不同阶段卵泡中的表达情况,并比较卵泡中其表达强度.结果:Glyoxalas I和UCH-L1在次级卵泡、成熟卵泡的颗粒细胞及卵泡膜细胞中呈强表达,表达强度高于其在原始卵泡及初级卵泡胞质中的表达;HSP27在原始卵泡及初级卵泡细胞质中呈强表达,表达强度高于其在在次级卵泡和成熟卵泡颗粒细胞及卵泡膜细胞中的表达;Glyoxalase I和HSP27在闭锁卵泡中呈强表达,表达强度高于其在生长卵泡中的表达;SAP在原始卵泡、初级卵泡、次级卵泡和成熟卵泡均表达,表达强度无明显差异;4种蛋白在白体中均不表达.结论:UCH-L1强表达和HSP27弱表达与卵泡成熟发育有关;Glyoxalase I强表达及HSP27强表达与卵泡闭锁及卵巢功能衰退有关.%Objective:To detect the expressions of ovarian function-related proteins in the ovarian follicles in different development stages,and to explore their roles and significances in the regulation of follicular development and ovarian function. Methods:The ovarian tissue containing primordial,primary,secondary follicles,mature follicles, atretic follicles and corpus albicans of the woman in the childbearing age was selected. Immunohisto chemical analysis was used to detect the expressions of Glyoxal enzyme I (glyoxalase I),ubiquitin carboxy-terminal hydrolase L1 (UCH-L1),heat shock protein 27 (HSP27),serum amyloid P (SAP)proteins in the ovarian follicles in different stages.The expression intensities of these proteins were compared.Results:The expression intensities of Glyoxalase I and UCH-L1 in the granulosa cells and theca cells of secondary follicles and marure follicles were strong,which were higher than those in the cytoplasm of primordial and primary follicles.The expression intensities of HSP27 in the cytoplasm of primordial and primary follicles were strong,which were higher than those in the granulosa cells and theca cells of secondary follicles and marure follicles.The expression intensities of Glyoxalase I and HSP27 in the atretic follicles were strong,which were higher than that in the growth follicles. The expressions of SAP were positive in the primordial,primary follicles,secondary and mature follicles and the expression intensities were not different.The expressions of four proteins in the corpus albicans did not express. Conclusion:The high expression of UCH-L1 and low expression of HSP27 are associated with the mature and development of follicles;the high expressions of Glyoxalase I and HSP27 are associated with the follicular atresia and ovarian failure.
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