Objective To analysis the expression of VEGF - C, NF -κB , bcl - 2 gene by inhibiting VEGF - C expression of tumor cells with RNA interference ( RNAi) technique. Methods According to the encoding sequence mRNA of human VEGF - C ,the target site for RNAi was designed,siRNA was transfected by liposome and the expression of VEGF -C,NF - κB,dc1 - 2 mRNA was further observed by RT - PCR. Results The expression of VEGF - C of HeLa cell lines treated with siRNA for 24h,48h was significantly inhibited, with in 24h 80.63% ±0.24% (P<0.001) and in 48h 38.9% ±0.85%(P<0. 01). The expression of NF - kB was also down - regulated in 24h,48h,with in 24h 37.55% ±2.76% (P<0.05) and in 48h 30.5% ±3.82% (P= 0.056). Thus the expression of bcl-2 was also inhibited in 24h,48h .with in 24h 76.95% ± 1. 91 % (P < 0.01) and in 48h 64. 11 % ±2. 96% ( P < 0. 05). Conclusion After being treated with VEGF - C siRNA of the HeLa cells, the expression of VEGF - C is effectively inhibited, NF - kB may be down - regulated and anti - apoptosis bcl - 2 gene is effectively inhibited.%目的 通过RNA干扰技术抑制宫颈癌细胞VEGF-C表达,探讨干扰后VEGF-C、NF - κB、bcl -2基因的表达.方法 根据人VEGF-C mRNA编码序列,设计RNA干扰的靶点,并用脂质体转染人宫颈癌HeLa细胞,通过RT - PCR法观察转染后肿瘤细胞VEGF-C、NF - κB 、bcl -2基因的变化.结果 转染siRNA 24h、48h可以使HeLa细胞VEGF-C mRNA含量降低,在24h降低80.63％ ±0.24％ (P＜0.001)；在48h降低38.9％±0.85％ (P＜0.01)；NF-κB mRNA含量也分别降低,在24h降低37.55±2.76％(P＜0.05)；在48h降低30.5％±3.82％ (P =0.056)；bcl - 2mRNA含量同时分别降低,在24h降低76.95％±1.91％,(P＜0.01)；在48h降低64.11％ +2.96％,(P＜0.05)).结论 脂质体介导的VEGF-C siRNA转染HeLa细胞后,可以有效抑制VEGF-C的表达；可能通过下调转录因子NF - κB,抑制抗凋亡基因bcl -2的表达.
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