Objective To study therapeutic effects of magnetic fluid hyperthermia induced by an alternating magnetic field on human carcinoma AS49 in nude mice. Methods Xenografted animal model of human lung cancer was established by innoculating human lung cancerA549 cells into BALB/c nude mice subcutaneously. Then the mice were randomly divided into 4 groups; control group, low dose group (67.5mg/ml) , medium dose group (90.0mg/ml)and high dose group(112. 5mg/ml) , respectively. Twenty four hours after magnetic fluid (0.2ml) was directly injected into the center of the tumor, the tumor was heated in an alternating magnetic field for 30 minutes. Tumor volumes were measured every week. The therapeutic effect was assessed by measuring the tumor volume and weight. Pathological examination was performed with light and electronic microscope after the treatment. Results Temperature of medium and high dosage group could reach 44. 5℃ and 46.8℃ , respectively. The tumor grew slowly in medium dosage and high dosage groups compared with the control group. The difference was significant ( P <0. 05 , respectively). Cytoclasis and apoptosis were observed under light microscope and electron microscope. Conclusion Magnetic fluid hyperthermia induced by AMF could inhibit the tumor growth and promote apoptosis of human being carcinoma A549 cells.%目的 探讨Fe304磁流体热疗对人肺癌A549裸鼠移植瘤生长的抑制作用.方法 将人肺癌A549细胞接种于裸鼠背部皮下建立移植瘤模型,当肿瘤长至直径(6 ～8mm)时,在预实验的基础上随机分为4组:对照组、低剂量(67.5mg/ml)组、中剂量(90.0mg/ml)组、高剂量(112.5mg/ml)组.3个实验组在注射0.2ml磁流体后24h,分别在交变磁场作用下作用30min,光纤传感器测量肿瘤内部和肛门的温度,每周测量肿瘤体积,观察磁流体热疗后肿瘤体积的变化、瘤体的病理变化,对肿瘤生长影响.结果 中、高剂量组的温度可以上升至有效治疗温度(＞42℃),热疗后第21天,与对照组比较,中、高剂量组瘤体的增长受到明显的抑制(P＜0.05),抑制效果与剂量呈剂量-效果依赖关系.组织学检查肿瘤细胞呈凋亡、坏死样改变.结论 磁流体热疗可以明显诱导人肺癌A549凋亡、导致坏死,抑制肺癌裸鼠移植瘤的生长.
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