The gathering problem appeared during the preparation of carboxymethyl chitosan nanoparticles ( CCN) was solved by antibacterial lipopeptide, and investigated the effect of it on the inhibitory activity of carboxymethyl chitosan nanoparticles. The CCN were prepared by emulsion-dispersed with antibacterial lipopeptide ionic crosslinking method and observed its stability by setting it still aside, and measured its viscosity changes by Ubbelohde viscometer, observed its morphological change by scanning electron microscope. The effect of different concentration of nanoparticles on the inhibition against Streptococcus aureus and E. coli were tested by turbidimetry. It was found that nanoparticles which were prepared by adding antibacterial lipopeptide were highly homogeneous and had good stability and low viscosity. It also significantly enhanced the inhibitory effect on S. aureus and E. coli. The results indicated that adding antibacterial lipopeptide could not only solve the gathering phenomenon in the preparation of the CCN but also improve its antibacterial effect.%利用抗菌脂肽解决羧甲基壳聚糖纳米粒制备过程中出现的团聚问题,并考察其对羧甲基壳聚糖纳米粒的抑菌效果影响.通过抗菌脂肽乳化分散离子交联法制备羧甲基壳聚糖纳米粒,静置观察其稳定性,乌氏粘度计测定其粘度变化,扫描电镜考察其形态改变,比浊法测定不同浓度纳米粒溶液对金黄色葡萄球菌和大肠埃希菌的抑制作用.实验发现,加入抗菌脂肽制备的纳米粒稳定性好,粘度降低,形态均一,并能显著增强对金黄色葡萄球菌和大肠埃希菌的抑制作用.结果表明,抗菌脂肽的加入能很好的解决纳米粒制备过程中出现的团聚现象,而且其抑菌效果也有很大改善.
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