目的：探讨血清脂质运载蛋白-2（LCN-2）和基质金属蛋白酶-9（MMP-9）在乳腺癌中的表达及临床意义。方法应用酶联免疫吸附法（ELISA）检测96例乳腺癌患者血清 LCN-2和 MMP-9的表达水平，并与42例乳腺良性病变患者和27例健康对照者比较，分析其与肿瘤大小、TNM 分期、分化程度、淋巴结转移以及 ER，PR表达的关系。结果乳腺癌患者血清 LCN-2表达量为89.76±28.38 ng／ml，明显高于乳腺良性病变组的56.44±21.15 ng／ml和健康对照组的44.32±16.84 ng／ml，差异均具有统计学意义（P＜0.05），而乳腺良性病变组与健康对照组无明显差异（P＞0.05）。乳腺癌患者血清 MMP-9的表达量为96.34±23.26ng／ml，明显高于乳腺良性病变组的28.16±11.57 ng／ml和健康对照组的23.54±13.61 ng／ml，差异均具有统计学意义（P＜0.05），而乳腺良性病变组与健康对照组无明显差异（P＞0.05）；相关分析显示，乳腺癌患者血清 LCN-2和 MMP-9水平之间呈正相关（r＝0.5135，P＜0.05）。乳腺癌患者血清 LCN-2和 MMP-9的表达水平与肿瘤大小、淋巴结转移情况、ER和PR表达、TNM分期明显相关（P＜0.05）；但与肿瘤分化程度无明显相关性（P＞0.05）。结论 LCN-2和 MMP-9可能在乳腺癌的发生、发展中起着重要作用，两者联合检测对于乳腺癌的辅助诊断、疗效监测及预后判断具有一定的临床意义。%Objective To evaluate the expression and its clinical significance of serum LCN-2 and MMP-9 in human breast cancer.Methods 96 patients with breast cancer,42 patients with benign breast diseases and 27 healthy controls were recrui-ted in this study.The LCN-2 and MMP-9 levels were measured by ELISA in the serum of 96 patients with breast cancer,42 patients with benign breast disease,and 27 health controls.Results The level of serum LCN-2 in the breast cancer group (89.76±28.38 ng/ml)was significantly higher than those in benign breast diseases (56.44±21.15 ng/ml)and healthy controls (44.32±16.84 ng/ml)(P<0.05).The level of serum MMP-9 in the breast cancer group (96.34±23.26 ng/ml) was significantly higher than those in benign breast diseases (28.16±11.57 ng/ml)and healthy controls (23.54±13.61 ng/ml)(P<0.05).No obvious difference in the levels of serum LCN-2 and MMP-9 was observed between benign breast disease and healthy controls (P>0.05).Correlation analysis showed that a positive correlation was found between the levels of serum LCN-2 and MMP-9 in breast cancer patients (r=0.513 5,P<0.05).The levels of serum LCN-2 and MMP-9 were significantly associated with tumor size,lymph node metastasis,the expression of ER and PR,TNM stage,but were not asso-ciated with tumor differentiation (P>0.05).The correlation analysis showed that the LCN-2 level was positive correlated with the MMP-9 level (P<0.05).Conclusion LCN-2 and MMP-9 could play a role in the tumorigenesis and development of breast cancer.Measuring of serum LCN-2 and MMP-9 may be a useful tool for diagnosis,effect monitoring and prognosis in breast cancer.
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