Objective To established the reference range of AMH in the serum by determinating the antt-Mullerian hormone level (AMH) in the serum and the relativity of reproductive age for healthy women of childbearing age in Handan area. Methods Used the Enzyme-linked Immunosorbent Assay (ELISA) to determine the AMH level of serum in the early follicle period (2~5 days menstrual cycle) or corpora luteum metaphase (4~8 days after BBT rised) .meanwhile,need to determine the six reproductive Hormone level of LH,FSH,T,E2,FRL and P for 165 cases with 20~50 years old healthy women of childbearing age. Results The AMH level of serum was distributed in the shape of abnormal status. So the reference range indicated in median (M) and scope. The AMH level of serum in the early follicle period and corpora luteum metaphase bdicated respectively 3. 87(0. 41~10. 69)ng/ml and 4. 64(0. 45—11. 64)ng/ml, which were not with the remarkable differences. The AMH level of serum in early stage and late stage indicated respectively 5.14(0.45~11.64)ng/ml and 2.02(0.41 —9. 03)ng/ml,which would with the remarkable differences (P< 0.001). The reference range of total level for AMH was 4. 55(0. 41~11. 65)ng/ml which will increase in inverse proportion to the age(r=—0. 365,P=0. 000). That was to say,as they grew older, the AMH level would reduce. Conclusion The medical reference range of AMH level in this thesis could be applied to health and medicine practice for clinical diagnosis and treatment of reproduction and infertility of women.%目的 通过测定邯郸地区健康育龄妇女血清抗苗勒氏管激素(AMH)水平及与生殖年龄的相关性,建立该地区实验室血清AMH参考值范围.方法 采用酶联免疫吸附技术(ELISA)检测165例20～50岁健康育龄妇女早卵泡期(月经周期2～5天)或黄体中期(BBT上升后4～8天)血清AMH水平,并同时检测LH,FSH,T,E2,PRL和P等六项生殖激素水平.结果 血清AMH水平呈非正态分布,故参考值范围用中位数(M)和范围表示.卵泡早期与黄体中期血清AMH水平分别为3.87(0.41～10.69)ng/ml和4.64(0.45～11.64 )ng/ml,差异无统计学意义(P=0.130)；育龄早期和育龄晚期血清AMH水平分别为5.148(0.45～11.64) ng/ml和2.02(0.41～9.03) ng/ml,差异有统计学意义(P＜0.001)；总体AMH水平参考值范围为4.55(0.41～11.65)ng/ml,且与年龄呈负相关关系(r=0.365,P=0.000),即随着年龄增长而减低.结论 AMH医学参考值范围可以用于妇女生殖和不孕症的临床诊断、治疗等医疗卫生实践的参考.
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