目的：探索葛根中有效成分葛根素的纯化方法，制备葛根提取物，提取物中葛根素含量应不得少于40％。方法通过对比试验确定树脂型号、用量及上样流速，用正交试验法确定树脂洗脱的条件。结果葛根经提取浓缩过滤后，上D-101大孔树脂吸附，1 g葛根药材（葛根素含量为2.71%）的提取液需用2.7 g D-101大孔树脂吸附，选择12 mL/min流速作为上样速度，选择20 mL/min水洗10倍柱体积的水清洗，以25%乙醇洗脱，收集洗脱液，浓缩至稠膏，减压干燥，所得葛根提取物葛根素含量均在40%以上。结论该法提取葛根中有效成分葛根素较稳定、可靠，适合工业生产。%Objective To explore the purification method of effective components pueraria in radix puerariae.The puer-aria in the extraction of radix puerariae shall not be less than 40%. Methods It was determined the type and amount of resin , sample flow rate by contrast testing and the resin elution conditions were confirmed by orthogonal testing method. Results The ex traction and concentration of radix puerariae after filtering ,D-101 macroporous resin was used to absorb. The extraction (2.71%) of 1 g radix puerariae was prepared and adsorbed with 2.7 g D-101 macroporous resin by 12 mL/min.Washing by water with 20 mL/min and 10-time column volume,using 25%ethanol elution,collecting the eluted liquid,concentrating,vacuum dry-ing,the pueraria extract of the puerarin content should be more than 40%. Conclusion This process abstracting the effective comporent,namely pueraria technique are stable and reliable being suitable for industrial production.
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