目的:探讨miRNA-302d在子宫内膜癌组织中的表达及其与临床病理参数的关系.方法:应用Re-al-time PCR方法检测42例子宫内膜癌组织及42例正常子宫内膜组织中miRNA-302d的相对表达量,并分析miRNA-302d在不同临床病理参数的子宫内膜癌组织中表达的差异性.结果:miRNA-302d在子宫内膜癌组织中的表达水平低于其在正常子宫内膜组织中的表达水平(P=0.020<0.05).手术分期为Ⅰ期与Ⅱ、Ⅲ、Ⅳ期的子宫内膜癌组织中miRNA-302d的表达存在统计学差异(P=0.034<0.05);在不同的肌层浸润深度(深度<1/2肌层、深度≥1/2肌层)的子宫内膜癌组织中 miRNA -302 d的表达具有统计学差异(P=0.006<0.05);而miRNA-302d在子宫内膜癌组织中的表达水平与组织学分化程度、淋巴转移及脉管浸润等临床病理参数之间无明显统计学关系(P>0.05).结论:miRNA-302d在子宫内膜癌组织中的表达明显低于其在正常子宫内膜组织中的表达,并且与不同手术病理分期(Ⅰ、Ⅱ、Ⅲ、Ⅳ期)、肌层浸润深度(深度<1/2肌层、深度≥1/2肌层)有关;提示其可能在子宫内膜癌组织中发挥抑癌基因的作用.%Objective:To investigate the expression of the miRNA-302d in endometrial cancer tissue,in order to explore its correlation with different clinical pathological factors.Methods:The qRT-PCR was used to detect the rel-ative expression of miRNA-302d in 42 cases tissue specimens of endometrial cancer and in 42 cases of normal endo-metrial tissues.The relationship between the expression level of miRNA-302d and the clinical pathological factors was analyzed.Results:The real-time PCR detected that the expression level of miRNA -302 d in endometrial cancer tissue was lower than that in normal endometrial tissue,and the difference was statistically significant(P=0.020<0.05).Moreover,the expression level of miRNA-302d was statistically different in endometrial cancer tissue of stage Ⅰ and stageⅡ,Ⅲ,Ⅳ(P=0.034<0.05).miRNA-302d expressed statistically differently in depth of infiltration of endometrial cancer tissue(P=0.006<0.05).However,miRNA-302d expression was not signifi-cantly associated with the other clinical pathological factors.Conclusion:The expression level of miRNA-302d was significantly lower in endometrial carcinoma tissues than that in normal endometrial tissues.And also,the expression level of miRNA-302d was extremely related with different surgical-pathological stages and the depth of infiltration of endometrial cancer tissue.It suggests that miRNA-302d may play a role in inhibiting cancer gene in endometrial cancer.
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