目的:应用逆转录实时荧光定量聚合酶链反应(real-time PCR)检测子宫内膜癌组织miR-367表达水平,并探讨不同病理分期、组织分化、肌层浸润深度、淋巴转移及脉管浸润等临床病理参数之间的关系及临床意义.方法:本实验分别收集了61例子宫内膜癌组织及56例正常子宫内膜组织,运用定量real-time PCR检测miR-367在子宫内膜癌组织中及正常子宫内膜组织的表达量的差异,并运用统计学方法分析miR-367与临床病理参数之间的相关性.结果:在子宫内膜癌组织及正常子宫内膜组织之间发现miR-367存在显著差异性表达,其中占85.25%的子宫内膜癌组织miR-367的表达水平低于正常内膜组织(P<0.05);在子宫内膜癌病例中,miR-367表达水平与肿瘤病理分期(FIGO分期)、组织分化程度及肌层浸润深度三种临床病理参数之间存在着统计学联系:miR-367低水平表达的病例通常表现出分化程度更低(P=0.001<0.05)和对肌层浸润程度更深(P=0.000 3<0.05)的特点.与年龄、淋巴转移、脉管浸润及远处转移无关(P>0.05).统计学分析,还得出在FIGO分期Ⅲ-IV期子宫内膜癌病例中miR-367的表达水平显著低于FIGO分期I、II期内膜癌(P<0.05).结论:在子宫内膜癌组织中miR-367在癌组织和正常组织之间有统计学差异;miR-367表达水平与子宫内膜癌临床病理参数中的病理分期、组织分化程度及肌层浸润深度有统计学差异,并且它可能作为子宫内膜癌发生及发展的生物学标志.%Objective:To detect the miR-367 expression level by the method of real-time PCR in endometrial cancer tissue,and explored their relationship with clinical pathological parameters in endometrial carcinoma,such as different pathological stages,different degrees of histological differentiation,infiltration depth,lymph node metastasis and vascular invasion,and discussed the clinical significance.Methods: We collected the data of 61 cases tissue specimens of endometrial cancer and 56 cases of normal endometrial tissue in the study.The RT-PCR was used to detect the miR-367 expression in those specimens and analyse the relationship between the expression level of miR-367 and the clinical pathological parameters.Results:The significant differences in the expression of miR-367 between the endometrial carcinoma and normal endometrial tissue,the expression level of miR-367 in 85.25% of cases was lower than that in normal endometrium(P<0.05).In the cases of endometrial carcinoma,there was a statistically significant relationship between miR-367 expression and tumor pathological stage(FIGO stage),histological differentiation degree and depth of muscular invasion.Cases of low level expression of miR-367 present a lower degree of histological differentiation(P=0.001<0.05)and a deeper degree of muscular infiltration degree(P=0.000 3<0.05) usually.Unrelated to age,lymph node metastasis,vascular invasion and distant metastasis(P>0.05).Through statistical analysis,we got that the expression level of miR-367 in the case of FIGO stage Ⅲ-IV endometrial carcinoma was significantly lower than that of FIGO stage I and II endometrial carcinoma(P<0.05).Conclusion:There was a statistically significant difference in the expression level of miR-367 in the endometrial carcinoma tissues and in the normal tissues.The expression level of miR-367 was significantly related to the pathological stage,histological differentiation degree,and the depth of muscular invasion in endometrial carcinoma.And it might serve as a biological marker for the genesis and development of endometrial carcinoma.
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