Objective:To investigate the effect of Ad-p53( p53 -expressing adenovirus)on the expression of p53 gene and the growth,cell cycle and apoptosis of MDA-MB-231 cells. Methods:The changes of human breast cancer cell line MDA-MB-231 morphology were observed by inverted microscopy. The apoptosis rate and cell cycle were analyzed by flow cytometry. Western blot was used to detect the protein expression of p53. Results:Significant changes of cell morphology in MDA-MB-231cells were observed by an inverted microscope. Flow cytometry re-vealed that Ad-p53 could increase the cell proliferation inhibition rate,and there was significant difference between MDA-MB-231 group and control group,and the proportion of MDA-MB-231 cells in S phase and G2/M phase decreased but increased in G0/G1 phase. Western blot results indicated that the p53 protein expression level in MDA-MB-231 cells increased obviously with Ad-p53. Conclusion:Ad-p53 can inhibit the cell growth and induce the cell apoptosis,which may be related to inhibition of the cell cycle and increase in the expression of wild-type p53 protein.%目的：观察重组人p53腺病毒（ p53-expressing adenovirus，Ad-p53）对人乳腺癌细胞MDA-MB-231细胞生长、细胞周期、凋亡和p53表达的影响。方法：流式细胞仪检测Ad-p53作用于MDA-MB-231细胞72h后细胞凋亡率和细胞周期变化，Western blot法检测MDA-MB-231细胞p53蛋白表达。结果：经Ad-p53处理72h后倒置显微镜下MDA-MB-231细胞形态发生明显的变化。流式细胞术检测结果显示， Ad-p53作用MDA-MB-231后其细胞凋亡率与对照组相比明显增加，G0/G1期细胞比例逐渐升高，S期、G2/M期细胞比例相应减少（ P＜0.05）。Western blot证实了外源野生型p53基因能在MDA-MB-231细胞表达增加。结论：Ad-p53可以抑制人乳腺癌细胞株MDA-MB-231的生长，促进其凋亡，其机制可能是通过阻滞细胞周期以及野生型p53蛋白表达增加实现的。
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