目的：检测乳腺癌及乳腺良性病变组织中hMAM-mRNA表达水平并探讨其表达特点及临床意义。方法：应用聚合酶链反应（ PCR）技术检测hMAM-mRNA在15例正常乳腺组织及15例乳腺增生组织中的表达情况。同时检测60例乳腺癌组织中hMAM-mRNA的表达。分析与肿块大小、年龄、腋窝淋巴结转移情况、免疫组化情况、病理学类型、肿瘤分期的关系。结果：正常乳腺组织中hMAM-mRNA阳性率33.33％（5/15），乳腺增生组织中阳性率53.33％（8/15），乳腺癌组织中阳性率83.33％（50/60）。60例乳腺癌患者中hMAM-mRNA表达与患者年龄、肿瘤大小、临床分期及淋巴结转移无关，与患者ER、PR、Her2状态无关。结论：hMAM-mRNA在乳腺癌组织中高表达，有可能成为乳腺癌微转移检测的分子标志物。%Objective:To explore hMAM-mRNA expression in breast cancer tissue. Methods:Using polymerase chain reaction( PCR)technique to detect hMAM-mRNA in 15 cases of normal breast tissue and 15 cases of hyper-plasia of mammary gland and 60 cases of breast cancer,and the relation with tumor size,age,axillary lymph node me-tastasis,immunohistochemical situation,pathological type,tumor staging. Results:In normal breast tissue hMAM -mRNA positive rate was 33. 33%(5/15),hyperplasia of mammary gland organization positive rate was 53. 33%(8/15),in 60 cases of breast cancer tissues hMAM-mRNA positive rate was 83. 33%(50/60). 60 cases of patients with breast cancer in hMAM mRNA expression and patient age,tumor size,clinical stage and lymph node metasta-sis,has nothing to do with the state of patients' ER,PR,and Her2 positive correlation. Conclusion:hMAM-mRNA expression in breast cancer is high,hMAM-mRNA maybe used for the detection of micrometastases in breast cancer tissue.
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