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首页> 外文期刊>Molecular medicine reports >Haptoglobin protein and mRNA expression in psoriasis and its clinical significance
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Haptoglobin protein and mRNA expression in psoriasis and its clinical significance

机译：牛皮癣中肝珠蛋白和mRNA的表达及其临床意义

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The present study aimed to explore the association between haptoglobin protein and mRNA expression and psoriasis. A total of 138 patients with psoriasis that were undergoing therapy at Linyi People's Hospital (Linyi, China) between January 2011 and January 2015 were enrolled in the present study. The mRNA expression levels of haptoglobin were detected by in situ hybridization; immunohistochemistry was used to detect haptoglobin protein expression; and double-labeling immunofluorescence was used to count Langerhans cells; western blotting was also conducted to determine protein expression. A receiver operating characteristic (ROC) curve was generated to assess the diagnostic value of haptoglobin for psoriasis. Compared with the normal and negative control (NC) groups, the mRNA expression levels of haptoglobin were markedly increased in the experimental group (P<0.05). Haptoglobin protein expression was also markedly increased in the experimental group compared with in the normal and NC groups (P<0.05). Conversely, there was no significant difference in haptoglobin expression between the NC group and the normal group (P>0.05). The critical value of haptoglobin mRNA in the diagnosis of psoriasis was 2.93, and sensitivity and specificity were 91.3 and 73.6%, respectively. The area under the ROC curve was 0.883 [95% confidence interval (CI)=0.837-0.929]. The critical value of haptoglobin protein in the diagnosis of psoriasis was 0.995, and sensitivity and specificity were 76.1 and 99.9%, respectively. The area under the ROC curve was 0.926 (95% CI=0.837-0.929). The present study demonstrated that the mRNA and protein expression levels of haptoglobin were increased in patients with psoriasis. Haptoglobin mRNA and protein expression were closely associated with the occurrence of psoriasis; therefore, haptoglobin may be considered a promising novel clinical indicator for the diagnosis of psoriasis.
机译：本研究旨在探讨触珠蛋白与mRNA表达和牛皮癣之间的关系。本研究共纳入2011年1月至2015年1月在临沂市人民医院（中国临沂）接受治疗的138例牛皮癣患者。原位杂交检测触珠蛋白的mRNA表达水平。免疫组化法检测触珠蛋白的表达;用双标记免疫荧光计数朗格汉斯细胞。还进行了蛋白质印迹分析以确定蛋白质表达。生成了一个接收器操作特征（ROC）曲线，以评估触珠蛋白对牛皮癣的诊断价值。与正常对照组和阴性对照组相比，实验组触珠蛋白的mRNA表达水平明显升高（P <0.05）。与正常组和NC组相比，实验组的肝红蛋白蛋白表达也显着增加（P <0.05）。相反，NC组与正常组的触珠蛋白表达无显着差异（P> 0.05）。触珠蛋白mRNA在银屑病诊断中的临界值为2.93，敏感性和特异性分别为91.3和73.6％。 ROC曲线下的面积为0.883 [95％置信区间（CI）= 0.837-0.929]。触珠蛋白在银屑病诊断中的临界值为0.995，敏感性和特异性分别为76.1％和99.9％。 ROC曲线下的面积为0.926（95％CI = 0.837-0.929）。本研究表明牛皮癣患者触珠蛋白的mRNA和蛋白表达水平升高。结合珠蛋白的mRNA和蛋白表达与牛皮癣的发生密切相关。因此，触珠蛋白可以被认为是诊断牛皮癣的有前途的新型临床指标。

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