Objective To observe the effects of magnetic stimulation on primary neuronal mi-gration in vitro.Methods Primary cultured SD rat cortical neurons were divided into four group:control group(group C),sham simulation group(group S),40% of maximum stimulation intensity group(0.4 T group),and 60% of maximum stimulation intensity group(0.6 T group).Cells were planted in the transwell chambers,and were stimulated from the second day to the sixth day after plantation.Moreover,cells in different groups were stained at the same time on the sixth day.Tr-answell chambers were inverted and cells were counted under light microscope.Results The numbers of migrated cells in 0.4T group(17.40±1.673)and 0.6T group(18.40±1.572)were significantly higher than those in group C(2.6±0.548)or group S(3.0±0.707)(P <0.05).The differences were not significant between group C and group S(P >0.05).Conclusion Magnetic stimulation can promote primary neuronal migration in vitro.%目的：体外观察磁刺激对原代神经元迁移的影响。方法原代培养 SD 大鼠皮质神经元，分为正常组（con-trol，C 组）、假刺激组（shame，S 组）、40％最大输出强度组（40％ of maximum intensity of stimulation，0．4T 组）、60％最大输出强度组（60％ of maximum intensity of stimulation，0．6T 组）。各组细胞接种于 transwell 小室内，自细胞接种后第2～6天接受磁刺激，连续刺激5 d，各组细胞于第6天同一时间染色，将 transwell 小室倒置，光学显微镜下计数。结果C 组有（2．6±0．548）个、S 组有（3．0±0．707）个细胞迁移，2组比较差异无统计学意义（P ＞0．05）；0．4T 组有（17．40±1．673）个、0．6T 组有（18．40±1．572）个细胞迁移，迁移的数量较 C 组及 S 组明显增多（P ＜0．05）。结论磁刺激可促进体外原代神经元迁移。
展开▼
