目的：研究乳腺癌外周血微转移与相应肿瘤组织微血管生成的关系，并探讨其临床意义。方法采用逆转录聚合酶链反应（RT-PCR）方法，对50例乳腺癌（乳癌组）及30例乳腺良性肿瘤患者（对照组）外周血中乳腺上皮黏蛋白（small breast epithelial mucin，SBEM）表达情况进行检测；采用免疫组织化学法，用 CD105标记肿瘤新生血管，计数乳腺癌和乳腺良性肿瘤组织微血管密度（MVD）；分析其相关性及与临床病理特征之间的关系。结果乳癌组 SBEM的阳性表达率为36％（18/50），高于对照组的0．0％（0/30）（P＜0．05）；乳癌组 MVD 值为24．56±4．12，高于对照组的8．61±2．87（P＜0．05）；乳癌组 SBEM表达与 MVD计数呈正相关（r＝0．712，P＜0．05），与TNM分期、淋巴结转移密切相关（P＜0．05）；乳癌组 MVD计数与肿瘤大小、淋巴结转移、TNM分期有关（P＜0．05）。结论乳腺癌外周血微转移与相应癌组织微血管密度密切相关，MVD较高者更易发生外周血微转移，并与肿瘤的发生、发展和转移有关。%Objective To investigate the correlation between peripheral blood micrometastasis and microvascular angiogenesis and its clinical significance in breast cancer.Methods The expres-sion of small breast epithelial mucin(SBEM)in peripheral blood was detected by RT-PCR in 50 patients with breast cancer (breast cancer group)and 30 patients with benign breast tumors (con-trol group).Furthermore,microvessel density (MVD)was counted by labeling new vessels with CD105 antibody.The relationships of MVD to SBEM and clinicopathologic features were ana-lyzed.Results Compared with control group,the positive SBEM expression rate and MVD value significantly increased in control group (36.0% vs 0.0% and 24.56±4.12 vs 8.61±2.87,respec-tively;P<0.05).The expression of SBEM was positively correlated with MVD in patients with breast cancer(r=0.712,P<0.05).In addition,SBEM expression was related to lymph node me-tastasis and TNM staging,and MVD count was related to tumor size,lymph node metastasis and TNM staging(P<0.05).Conclusion The micrometastasis in peripheral blood is closely correla-ted with microvascular angiogenesis in breast cancer.Patients with higher MVD were more likely to have micrometastasis in peripheral blood.The MVD is correlated with the occurrence,develop-ment and metastasis of breast cancer.
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