目的：通过检测妊娠期糖尿病（GDM）孕妇血清、脐血、胎盘组织中纤溶酶原激活物抑制剂-1（PAI-1）、脂联素（AP）、白介素-1（IL-1）的表达水平，探讨其与 GDM 发生、发展的关系。方法选择50例 GDM 孕妇为试验组，50例正常孕妇为对照组。采用 ELISA 法测定2组孕妇血清与脐血 PAI-1、AP、IL-1表达水平，采用实时定量 PCR测定2组胎盘组织中 PAI-1、AP、IL-1表达水平。结果试验组血清、脐血、胎盘组织 AP 表达水平均较对照组显著降低（P ＜0．05），而 PAI-1、IL-1表达水平均较对照组显著升高（P ＜0．05）。结论AP 的低表达、PAI-1与 IL-1的高表达可能与 GDM 的发生、发展有关。%Objective To investigate the relationships ofthe levels of plasminogen activator in-hibitor-1(PAI-1),adiponectin(AP)and interleukin-1(IL-1)expression in maternalserum,umbili-cal cord blood andplacental tissue to the occurrence and development of gestational diabetes melli-tus(GDM).Methods The expression levels of PAI-1,AP and IL-1 in maternal serum and umbili-cal cord blood were measured by ELISA and those in placental tissue were detected by real-time PCR in 50 GDM women(experimental group)and 50 normal pregnant women(control group).Re-sults Compared with control group,AP expression decreased but PAI-1 and IL-1 expression in-creased in maternal serum,umbilical cord blood and placental tissue in experimental group(P <0.05).Conclusion The down-regulation of APexpression and the up-regulation of PAI-1and IL-1 expression may be involved inthe occurrence and development of GDM.
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