目的 研究CD基因修饰神经干细胞(NSCs)对脑胶质瘤细胞的抑制作用、效果及机制,为NSCs在脑胶质瘤治疗中的应用提供理论依据.方法 以胎脑作为供体,采用神经球形成法培养NSCs,脂质体介导CD基因转染NSCs,G418筛选.NSCs/CD与C6胶质瘤细胞分组共培养,MTT检测细胞存活情况.结果 成功构建CD基因转染NSCs,NSCs/CD对C6细胞生长有明显抑制作用.结论 NSCs是良好的载体,NSCs/CD-5-FC自杀基因治疗系统具有很强的抗肿瘤效果.%Objective To investigate the inhibition effect of cytosine deaminase (CD) gene-modified neural stem cells (NSCs) and the action mechanism, and to provide a theoretical basis for the treatment of glioma. Methods Neurosphere-forming NSCs were isolated from fetal brain. CD gene was introduced into NSCs by liposome-mediated transfection and placed under G418 selection. NSCs/CD and C6 glioma cells were co-cultured and cell survival was assessed with MTT assay. Results CD gene-modified NSCs were successfully constructed. NSCs/CD significantly inhibited the growth of C6 cells. Conclusion NSCs are good carrier cells and NSCs/CD-5-FC suicide gene therapy system has a strong anti-tumor effect.
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