Sonodynamic cytotoxic effect of hematoporphyrin monomethyl ether on C6 glioma cells - in vitro study

机译：血卟啉单甲醚对C6胶质瘤细胞的声动力学细胞毒性作用-体外研究

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Objectives: To study the cytotoxicity induced by photosensitizer mediated sonodynamic effects on c6 glioma cells in vitro and to lay the experimental foundation for the sonodynamic therapy (SDT) of glioma tumor. Methods: A domestic photosensitizer hematoporphyrin monomethyl ether (HMME) was used as the sensitizer. The growth inhibition rate of c6 glioma cells at different times after SDT was investigated by MTT assay. The apoptotic rate and cell circle profiles of c6 glioma cells was examined by a flow cytometry. Results: The growth inhibition rate of c6 glioma cells after SDT significantly increased. Ultrasound only group also showed some effects. Whereas, the inhibition rate of HMME only group has no significant difference compared with the control group (without ultrasound and HMME). HMME-mediated SDT increased the apoptotic rate and the percentage of S phase cells but decreased the percentages of G0/G1 and G2/M. SDT also increased the apoptosis and proliferation rate (APR). Conclusions: Our preliminary data indicated that SDT could kill c6 glioma cells in vitro and possibility through induction of apoptosis and arrest cell circle at S/G_2.

机译：目的：研究光敏剂介导的声动力学对C6胶质瘤细胞的体外细胞毒性作用，为胶质瘤肿瘤的声动力学治疗（SDT）奠定实验基础。方法：采用国产光敏血卟啉单甲醚（HMME）作为敏化剂。用MTT法研究了SDT后不同时间c6胶质瘤细胞的生长抑制率。通过流式细胞术检查了c6神经胶质瘤细胞的凋亡率和细胞环图。结果：SDT后c6胶质瘤细胞的生长抑制率明显增加。仅超声组也显示出一些效果。然而，仅HMME组的抑制率与对照组（无超声和HMME）相比无显着差异。 HMME介导的SDT提高了细胞凋亡率和S期细胞的百分比，但降低了G0 / G1和G2 / M的百分比。 SDT还增加了细胞凋亡和增殖率（APR）。结论：我们的初步数据表明，SDT可以在体外杀死c6胶质瘤细胞，并可能通过诱导凋亡和将细胞圈阻滞在S / G_2上来杀死。

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《Biophotonics and Immune Responses II; Progress in Biomedical Optics and Imaging; vol.8 no.15; Proceedings of SPIE-The International Society for Optical Engineering; vol.6438》|2007年|64380I.1-64380I.6|共6页
会议地点 San JoseCA(US)
作者
Wu Yue; Da-yong Song; Zhao Zhao; Shao-dong Cao; Xiang-xi Meng; Xu Zhang; Zheng Huang;
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作者单位

Department of Neurosurgery, The Fourth College Hospital Haerbin Medical University, Harbin, P. R. China;

Department of Neurosurgery, The First College Hospital Haerbin Medical University, Harbin, P. R. China;

Department of Radiation Oncology, Colorado Unive;

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原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;
关键词
sonodynamic therapy; SDT; HMME; apoptosis;

机译：声动力学治疗； SDT； HMME;凋亡;

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专利

1. Calcium Overload and in vitro Apoptosis of the C6 Glioma Cells Mediated by Sonodynamic Therapy (Hematoporphyrin monomethyl ether and ultrasound) [J] . Hao Dongning, Song Yanbin, Che Zhen, Cell biochemistry and biophysics . 2014,第2期

机译：声动力学疗法（血卟啉单甲醚和超声）介导的钙超载和C6胶质瘤细胞的体外凋亡
2. In vitro stimulation of calcium overload and apoptosis by sonodynamic therapy combined with hematoporphyrin monomethyl ether in C6 glioma cells. [J] . Shaochun Dai, Changqing Xu, Ye Tian, Oncology letters . 2014,第4期

机译：声动力学疗法联合血卟啉单甲醚体外刺激C6胶质瘤细胞中钙超载和细胞凋亡。
3. Apoptotic effect of sonodynamic therapy mediated by hematoporphyrin monomethyl ether on C6 glioma cells in vitro. [J] . Dai S, Hu S, Wu C Acta Neurochirurgica . 2009,第12期

机译：血卟啉单甲醚介导的声动力疗法对C6神经胶质瘤细胞的体外凋亡作用。
4. Sonodynamic cytotoxic effect of hematoporphyrin monomethyl ether on C6 glioma cells - in vitro study [C] . Wu Yue, Da-yong Song, Zhao Zhao Shao-dong Cao, Conference on Biophotonics and Immune Responses . 2007

机译：血流动力学对血卟啉单甲醚对C6胶质瘤细胞的疗效 - 体外研究
5. An in vitro investigation of the responses of C6 rat astroglioma cells to interleukin-1(beta) and tumor necrosis factor alpha. [D] . Dotson, Robert Steven. 1998

机译：体外研究C6大鼠星形胶质瘤细胞对白介素1β和肿瘤坏死因子α的反应。
6. In vitro stimulation of calcium overload and apoptosis by sonodynamic therapy combined with hematoporphyrin monomethyl ether in C6 glioma cells [O] . SHAOCHUN DAI, CHANGQING XU, YE TIAN, -1

机译：声动力学疗法联合血卟啉单甲醚体外刺激C6神经胶质瘤细胞钙超载和凋亡
7. Calcium Overload and in vitro Apoptosis of the C6 Glioma Cells Mediated by Sonodynamic Therapy (Hematoporphyrin monomethyl ether and ultrasound) [O] . 2014

机译：声动力学疗法（血卟啉单甲醚和超声）介导的钙超载和C6胶质瘤细胞的体外凋亡

Sonodynamic cytotoxic effect of hematoporphyrin monomethyl ether on C6 glioma cells - in vitro study

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