Objective To observe the effects of dendrobium compound on the apoptosis and mRNA expression of bax and bcl-2 in islet cells in diabetes rats. Methods The diabetes was induced by high fat and glucose feeding for 1 month,followed by intraperitoneal injection of strepto-zotocin (30 mg). The diabetes rats were treated with dendrobium compound (7. 5, 15. 0, 30. 0 g · kg-1)for two weeks and the apoptosis and Mrna expression of bax and bcl-2 were detected in islet cells. Results Dendrobium compound significantly decreased fasting blood glucose concentrations,islet cell apoptosis and bax expression, but obviously increased bcl-2 expression (P<0. 05 or P<0. 01). Conclusion Dendrobium compound can reduce blood glucose concentrations and islet cell apoptosis through decreasing bax expression and increasing bcl-2 expression in pancreatic tissue in diabetes rats.%目的 研究石斛合剂对高糖、高脂+STZ造模糖尿病大鼠胰岛细胞凋亡及相关基因Bax、Bcl-2 mRNA表达的影响.方法 大鼠高脂、高糖饲养1个月后腹腔注射STZ(30 mg)制备糖尿病模型,经石斛合剂(7.5、15.0、30.0 g·kg-1)治疗2周后检测胰岛细胞凋亡及凋亡相关基因Bax、Bcl-2 mRNA的表达.结果 石斛合剂可显著地降低糖尿病大鼠空腹血糖(P<0.05或P<0.01);石斛合剂可显著减少糖尿病模型大鼠胰岛细胞凋亡(P<0.05),降低Bax mRNA表达(P<0.05),增加Bcl-2 mRNA表达(P<0.05或P<0.01)).结论 石斛合剂可降低血糖、减少大鼠胰岛细胞凋亡,可能与其降低糖尿病模型大鼠胰腺组织Bax、增加Bcl-2表达有关.
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