Objective To detect the expression of serum IL-6,IL-10 and TNF-a in children with Mycoplasma pneumonia and their relationship with disease severity. Methods Serum IL-6, IL-10 and TNF-a were detected using double sandwich ELISA in 60 children with acute phase of Mycoplasma pneumoniae (acute phase group) ,52 children with convalescence phase of Mycoplasma pneumoniae (convalescence phase group) and 30 healthy children (control group). The relationship between serum IL-6,IL-10 and TNF-a levels and disease severity was investigated. Results Compared with acute phase group, serum IL-6 and TNF-a levels significantly decreased and IL-10 levels obviously increased in convalescence phase group and control group(P<0. 05). There were no significant differences in serum IL-6, IL-10 and TNF-a levels between convalescence phase group and control group (P>0. 05). In acute phase group, serum IL-6 and TNF-a levels significantly increased and IL-10 levels obviously decreased in children with CPIS>6 compared with children with CPIS<6 (P<0. 05 or P<0. 01). Conclusion Cytokine IL-6,IL-10 and TNF-a are involved in the pathogenesis of Mycoplasma pneumonia and are correlated with disease severity in children. The levels of IL-6,IL-10 and TNF-a can be used as indexes for evaluating the progrnnosis and treatment of Mycoplasma pneumonia.%目的 探讨白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)在小儿支原体肺炎的表达及其与病情严重程度的关系.方法 选择60例支原体肺炎急性期患儿(急性期组)、52例支原体肺炎恢复期患儿(恢复期组)为研究对象,同时选择30例健康儿童为对照组.采用双夹心ELISA法检测3组IL-6、IL-10、TNF-α并探讨其与疾病严重程度的关系.结果 支原体肺炎急性期患儿血清IL-6及TNF-α水平显著升高,血清IL-10显著降低,与支原体肺炎恢复期患儿及健康儿童比较差异有统计学意义(均P＜0.05)；支原体肺炎恢复期患儿及健康儿童血清IL-6、IL-10、TNF-α差异无统计学意义(均P＞0.05).支原体肺炎急性期患儿中CPIS评分＞6分者与CPIS评分≤6分者比较,血清IL-6及TNF-α水平显著增高,IL-10水平显著降低(P＜0.05或P＜0.01).结论 细胞因子IL-6、IL-10、TNF-α参与小儿支原体肺炎的发病过程,且与病情严重程度有关,可作为临床判断预后及治疗的指标.
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