Objective To investigate the effects of Xinmailong on myocardial injury in asphyxiated neonatal rats after hypoxia-ischemia and its mechanism through observing the influence of Xinmailong on the expression of NF-κB and TNF-α in myocardium. Methods A total of 96 neonatal Sprague-Dawley rats were randomly divided into three groups: control group (n=32), asphyxia group(n=32) and Xinmailong group(n=32). Rats were tested at various time points (3,6,12 and 24 hours; 8 rats per time point per group). The newborn rat model of asphyxia was induced in asphyxia group and Xinmailong group. The levels of CK-MB and the pathological changes in myocardial tissue were observed. The expression of NF-κB in the myocardium was detected by immunohistochemistry and the levels of TNF-α were determined by ELISA. Results The levels of CK-MB and the expression of NF-κB and TNF-α in asphyxia group was higher than that in control group (P＜0. 01). The highest levels were observed at 6 hours,and there was a positive correlation between NF-κB and TNF-α(r=0. 979, P＜0.01). Compared with asphyxia group, Xinmailong significantly decreased the levels of CK-MB and the expression of NF-κB and TNF-α(P＜0.01). Conclusion The expression of NF-κB and TNF-α increases in myoeardium of asphyxiated neonatal rats and reaches the highest levels at 6 hours. Xinmailong can protect the immature myocardium by reducing the expression of NF-κB and TNF-α in newborn rats with myocardial injury.%目的 观察窒息新生鼠应用心脉隆干预后心肌缺氧缺血损伤NF-κB和TNF-α的变化,探讨心脉隆抗未成熟心肌缺氧缺血损伤的作用及其机制.方法 将96只新生SD大鼠按随机数字表法分为3组:正常对照组(n=32)、窒息组(n=32)、心脉隆干预组(n=32),各组再分3、6、12、24 h 4个时间点,每个时间点各8只;建立新生鼠窒息模型,测定新生鼠血清心肌酶(CK-MB)水平并观察心肌组织病理形态学变化,应用免疫组织化学法检测心肌NF-κB的表达、ELISE法检测心肌TNF-α的表达.结果 窒息组血清CK-MB水平、心肌NF-κB和TNF-α的表达较正常对照组显著增强,以6 h达到高峰(均P＜0.01),且心肌NF-κB和TNF-α表达具有正相关性(r=0.979,P<0.01);心脉隆干预组与窒息组相比,血清CK-MB水平、心肌NF-κB和TNF-α的表达均显著减弱(均P＜0.01).结论 窒息新生鼠心肌组织NF-κB、TNF-α的表达增加,窒息6 h后NF-κB、TNF-α的表达达高峰;心脉隆可降低心肌NF-κB、TNF-α的表达,减轻未成熟心肌损伤.
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