Objective To investigate a newer method for treatment of Parkinson's disease (PD).Methods A newer method of immunoisolation (microencapsulation) was utilized for encapsulation of dopamine-secreting PC12 cells, and the cells were implanted into the brains of Parkinson's disease rats.And then, the change of apomorphine-induced rotational behavior in PD rats were observed. The quantities of dopamine (DA) in the area of transplantation were evaluated by high performance liquid chromatography with electrochemical detection (HPLC-EC). Tyrosine hydroxylase (TH) antibody immunohistochemistry stains of the cells in the implanted area were also proceeded. Results The apomorphineinduced rotational behavior of PD rats reduced significantly, the quantities of DA in the implanted area were increased much more than the control area at the different times. At the same time, the lethal tumor formation was observed in unencapsulated PC12 cells implantation. The microcapsules in the PD rats brains were intact and TH immunopositive PC12 cells could exist for 3 months postimplantation.Conclusions Implantation of microencapsulated PC12 cells for treatment of Parkinson's disease may be a new and effective method that can avoid the shortcoming of agents, stereotactic therapy, and implantation of embryonic brains.%目的研究治疗帕金森病的一种新方法.方法采用免疫隔离技术-微囊技术将能分泌多巴胺的PC12细胞包裹后,移植到帕金森病大鼠脑内,观察阿朴吗啡诱发病鼠旋转行为变化,高效液相色谱电化学法测定移植区多巴胺含量,酪氨酸羟化酶(TH)抗体免疫组织化学染色观察移植区阳性细胞表达.结果帕金森病大鼠阿朴吗啡诱发旋转行为明显改善,不同时相点的移植区多巴胺含量较对照组明显升高,而未行微囊化的PC12细胞移植则会有致死性肿瘤形成.移植后3月,大鼠脑内微囊完整,仍有TH阳性PC12细胞存活.结论微囊化PC12细胞移植,可避免药物治疗、立体定向毁损手术、胎脑移植等方法的诸多弊端,因此是一种新的有效的治疗帕金森病的方法.
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