Objective:To explore the expression of CD4 +CD25 +regulatory T cells and their relevant factors in pleural effusion and peripheral blood of patients with malignant tumors.Methods:Forty-two patients with malignant pleural effusion were selected as the research group,and 38 healthy people were selected as the control group.The CD4 +CD25 + /CD4 + proportion,lymphocyte subsets,lev-els of transforming growth factor-β1 (TGF-β1 ),interleukin-10 (IL-10)and interferon-γ(IFN-γ)in pleural effusion and peripheral blood in the research group as well as peripheral blood in the control group were detected using flow cytometry (FCM)and enzyme linked immunosorbent assay (ELISA).Results:The CD4 +CD25 + /CD4 + proportion,levels of TGF-β1 and IL-10 in pleural effusion and peripheral blood in the research group were significantly higher than those in the control group (P <0.01 ),but all the above mark-ers in pleural effusion were prominently higher than those in peripheral blood (P <0.01 ).In the research group,the levels of IFN-γin pleural effusion and peripheral blood were notably lower than those in control group,in which those in pleural effusion were lower (P <0.01 ).Conclusion:Detection of CD4 +CD25 + regulatory T cells and levels of TGF-β1 ,IL-10 and IFN-γin pleural effusion and periph-eral blood of patients with malignant tumors is conductive to judging the disease prognosis and outcome.%目的：探讨 CD4＋CD25＋调节性 T细胞及其相关因子在恶性肿瘤患者胸腔积液及外周血中的表达。方法：选取42例恶性肿瘤胸腔积液患者为研究组，38例健康体检者为对照组，采用流式细胞仪（FCM）和酶联免疫法（ELISA）检测研究组胸腔积液和外周血、对照组外周血中 CD4＋CD25＋ ／CD4＋比例、淋巴细胞亚群以及转化生长因子-β1（TGF-β1）、白细胞介素-10（IL-10）和干扰素-γ（IFN-γ）水平。结果：研究组胸腔积液及外周血中 CD4＋CD25＋ ／CD4＋比例及 TGF-β1、IL-10水平均显著高于对照组（P ＜0．01），且胸腔积液高于外周血（P ＜0．01），而 IFN-γ水平显著低于对照组，且胸腔积液低于外周血（P ＜0．01）。结论：对恶性肿瘤患者外周血和胸腔积液中 CD4＋CD25＋调节性 T 细胞及 TGF-β1、IL-10和 IFN-γ水平进行检测有利于判断病情预后和转归。
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