在基础日粮(对照组)中分别添加2%由不同比例的刺五加(Acanthopanax senticosus Harms)、枸杞子(L.Chinense Mill.)、金银花(Lonicera japonica Thunb)和黄芪(Astragalus membranaceus)配伍的复方中草药,记为复方Ⅰ、复方Ⅱ、复方Ⅲ、复方Ⅳ,共四个处理组,饲喂镜鲤幼鱼(Cyprinus carpio var.specularis),并分别于第14、28及42天检测其血清中补体C3的含量、血清及免疫器官溶菌酶、NBT阳性细胞及巨噬细胞吞噬能力的活性,以研究本复方中草药对其非特异性免疫功能的影响.结果表明,14 d时,复方Ⅰ组血清溶菌酶活性极显著高于对照组(P＜0.01);28 d时,复方Ⅰ、Ⅲ组显著高于对照组(P＜0.05).14 d时,复方Ⅰ-Ⅲ组脾脏溶菌酶活性极显著高于对照组(P＜0.01);28 d时,复方Ⅱ组显著高于对照组(P＜0.05).28、42 d时,各复方组NBT阳性细胞数显著高于对照组(P＜0.05).14和28 d时,复方Ⅰ组补体C3含量极显著高于对照组(P＜0.01);42 d时,各复方组极显著高于对照组(P＜0.01).14 d时,复方Ⅰ、Ⅳ组的巨噬细胞吞噬活性极显著高于对照组(P＜0.01);28 d时,各复方组显著高于对照组(P＜0.05);42 d时,复方Ⅰ组极显著高于对照组(P＜0.01).试验结果显示本试验中的四组复方中草药分别按质量分数为2%的量加到镜鲤幼鱼饲料中,可不同程度提高镜鲤幼鱼的非特异性免疫能力,其中复方Ⅰ组效果最佳.%2％ Chinese herbals compounds of different proportion (Acanthopanax senticosus Harms, L. chinense Mill, Lonicera japonica Thunb and Astragalus membranaceus) were supplemented to the basical formulated diet (control group). Four treatment groups were set up to feed Cyprinus carpio var. specularis Juvenile fish. The content of C3 in serum and the activities of NBT positive cells and phagocytosis of phagocyte and lysozyme in serum and immun organs were measured respectively on the 14th, 28th and 42nd day, with the view of studying on the influence of compound Chinese medicine to its nonspecific immunity. The results showed that the activities of lysozyme in serum of compound Ⅰ had very significant difference with the control group (P＜0.01) on the 14th day, and significantly higher than it; The compounds Ⅰ and Ⅲ had significant difference with the control group (P＜ 0.05) on the 28th day. The activities of lysozyme in head kidney of compounds Ⅲ and IV had very significant difference with the control group (P＜0.01) on the 42nd day. The activities of lysozyme in spleen of compoundsl-III had very significant difference with the control group (P＜0.01) on the 14th day; The compound ⅠI had significant difference with the control group (P＜0.05) on the 28th day. The NBT positive cells of the compound groups had significant difference with the control group (P＜0.05)on the 28th and 42nd day. The content of complement C3 of compound Ⅰ and Ⅱ had very significant difference with the control group (P＜0.01) on the 14th day; The compound Ⅰ and Ⅲ had very significant difference with the control group (P＜0.01) on the 28th day; The compound groups had very significant difference with the control group (P＜0.01) on the 42nd day. The phagocytosis percentage and phagocytic index of sheep red blood cells made by macrophage of compound land IV had very significant difference with the control group (P＜0.01) on the 14th day; The compound groups had significant difference with the control group (p＜0.05) on the 28th day; The compound Ⅰhad very significant difference with the control group (P＜0.01) on the 42nd day. The study showed that the Chinese herbal compounds could be added to feed of the Cyprinus carpio var. specularis to increase the activities of non specific immunity, but long-time feeding could produce toxic effects, it was suggested that medication time was 28 d, the most effective compound was compound Ⅰ.
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