Root rot is one of an A stragalus membranaceus disease seriously affecting A stragalus membranaceus production . The main pathogen are Fusarium solani and Fusarium oxysporum . In this study ,the root of Astragalus membranaceus obtained from Gansu Longxi are isolated with pathogens , and then tested its pathogenicity through tissue isolation , root inoculation , pot experiment . Finally , obtained 5 strains of A stragalus membranaceus root rot pathogens , stain G2 , G3 and G4 are identified to Fusarium solani , strain G5 and G6 are identified to Fusarium ox ysporum by culture characteristics , microscopic morphology and rDNA ITS sequences analysis .%黄芪根腐病是严重影响黄芪产量的病害之一，为确定其致病的主要致病菌，本实验对采自甘肃陇西的黄芪根腐病病株以组织分离、离体根部接种、盆栽实验法进行病原菌的分离和致病性测定。结果获得了5株黄芪根腐病致病菌，并通过培养特征、显微形态及 rDNA ITS序列分析综合鉴定，确定菌株G2、G3、G4为茄腐镰刀菌（Fusarium solani），G5、G6为尖孢镰刀菌（Fusarium xoysporum）。
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