A method for determination of total flavonoids in Hippophe rhamnoids L leaves is established , by singlefactor screening , multiple‐factor orthogonal test and methodology examination of NaNO2‐Al (NO3 )3‐NaOH system spectrophotometry . The single factor includs reaction volume and reaction time .The results show that the optimization methods are :Take 1 mL sample solution in the beaker ,add 70% ethanol 1 mL and 5% NaNO2 0.5 mL ,shake well ,react 6 min .Then add 10% Al(NO3)3 0.5 mL , shake well , react 6 min ;After that add 4% NaOH 6 mL , shake well , react 15 min , determine absorbance at 510 nm with 70% ethanol as reference solution . The methodology shows that the method has good stability and reproducibility , high precision and recovery . It is more suitable for the determination content of total flavonoids in Hippophe rhamnoids L leaves ,which has simple equipment , convenient operation and high accurate . using this method , the content of total flavonoids is 2.049% , which is 1.337 times than before optimized . Both of then reach to the regulation standard of “China pharmacopoeia” . This method can be looked as a reference method for the determination of total flavonoids in other materials .%本研究通过比色条件NaNO2，Al（NO3）3和NaO H的加入量和静置时间单因子筛选、多因子正交试验和方法学考察，建立了NaNO2‐Al（NO3）3‐NaO H体系分光光度法测定沙棘叶总黄酮含量的优化方法．结果得出测定沙棘叶总黄酮含量的步骤是：取1 mL样液于烧杯中，加70％的乙醇1 mL ，再加入5％的亚硝酸钠溶液0.5 mL ，摇匀，静置6 min ；再加入10％的硝酸铝溶液0.5 mL ，摇匀，静置6 min；然后加入4％的氢氧化钠溶液6 mL ，摇匀，放置15 min后，以70％乙醇作参比溶液，于波长510 nm 处测定吸光值．测得沙棘总黄酮含量为2.049％，比优化前提高1.337倍，均达到《中国药典》规定的标准．该法稳定性和重现性好，精密度和回收率高，并且设备简单、操作便捷，可为其他材料总黄酮的测定提供参考．
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