The growth curve assay, FCM analysis, AO/EB double staining and single cell gel electrophoresis are used to investigate the proliferation inhibition, cell cycle arrest, apoptosis induction and DNA damage of damnacanthal on human leukemia HL-60 cells respectively. The results demonstrate that damnacanthal exhibits significant cell proliferation inhibition for human promyelocytic leukemia HL-60 cells at concentration of 2.5- 12.5 fig ? mL"1 ; it induces cell cycle arrest in Gj, S or G2/M phase separately according to different concentrations range from 5 ~40 ^g'mL"1 ; the compound also induces significant apoptosis in HL-60 cells with 24~48 h incubation time and 2. 5~12. 5 jug?mL~: concentrations, the ratio of apoptotic cells is in a time-and dose-dependent manners; the comet-like cells rate of treated groups(5 ~ 12. 5 /izg'mL"1) is significantly different from the control group, this indicates that the compound exerts a serious damage on cell nucleus and DNA. The effect of DNA damage which induces by damnacanthal in HL-60 cells, in conclusion, is probably a reason causing cell cycle arrest, cell proliferation inhibition and cell apoptosis.%利用生长曲线法、流式细胞术、AO/EB双染法及单细胞凝胶电泳法,分别检测了蒽醌化合物虎刺醛对人早幼粒白血病细胞HL-60的生长抑制、细胞周期阻滞、细胞凋亡诱导以及DNA损伤能力.结果表明,2.5～12.5μg·mL-1的虎刺醛对人早幼粒白血病细胞HL-60具有显著的生长抑制作用；不同浓度(5～40 μg·mL-1)的虎刺醛,可引起HL-60细胞G1期、S期和G2/M期周期阻滞；在2.5～12.5 μg·mL-1浓度范围和24～48 h培养时间下,虎刺醛可不同程度诱导HL-60细胞,其凋亡率达到显著水平(P＜0.05)或极显著水平(P＜0.01),且具有时间和浓度依赖性关系；与对照组相比,药物处理组(5～12.5 μg·mL-1)均有极显著性(P＜0.0l)的彗星率,这表明,该浓度条件下,虎刺醛可较大程度地导致细胞核DNA损伤,这可能是引起周期阻滞进而抑制细胞增殖,最终导致细胞凋亡的原因.
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