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首页> 外文期刊>Life sciences >POTENT GROWTH INHIBITORY ACTIVITY OF A NOVEL ORNITHOGALUM CHOLESTANE GLYCOSIDE ON HUMAN CELLS - INDUCTION OF APOPTOSIS IN PROMYELOCYTIC LEUKEMIA HL-60 CELLS
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POTENT GROWTH INHIBITORY ACTIVITY OF A NOVEL ORNITHOGALUM CHOLESTANE GLYCOSIDE ON HUMAN CELLS - INDUCTION OF APOPTOSIS IN PROMYELOCYTIC LEUKEMIA HL-60 CELLS

机译：新颖的原核胆甾醇糖苷对人细胞的潜在生长抑制作用-早幼粒白血病HL-60细胞的凋亡诱导。

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摘要

Growth inhibitory activities of a novel 22-homo-23-norcholestane glycoside found in bulbs of Ornithogalum saundersiae were examined in vitro using human promyelocytic leukemia HL-60 cells, human T-lymphocytic leukemia MOLT-4 cells, and mitogen-stimulated human peripheral-blood mononuclear cells (PBMC). The growth of HL-60 cells and MOLT-4 cells was strongly suppressed in the presence of the glycoside; the IC(50)s of which were 21.0 and 18.0 nM, respectively. Suppressive effect of the glycoside on HL-60 cell growth appears to be mediated partially through induction of apoptosis which was demonstrated by the presence of DNA fragmentation of the leukemic cells. Flow cytometric analysis of glycoside-treated HL-60 cells also demonstrated apoptotic cells with low DNA content and showed a decrease of G0/G1 cells and a concomitant increase of S and/or G2/M cells. The growth inhibiting effect of the glycoside on HL-60 cells was promoted by calcium and was inhibited in the presence of zinc, which support involvement of endonuclease activation in the glycoside-induced apoptosis. The glycoside also inhibited mitogen-stimulated blastogenesis of PBMC, the IC50 of which was 6.2 nM. These results provided the first evidence ever for the potent growth inhibitory activity of Ornithogalum glycoside on human leukemia cell lines and PBMC. [References: 22]
机译：使用人早幼粒细胞白血病HL-60细胞，人T淋巴细胞白血病MOLT-4细胞和促有丝分裂原刺激的人外周血单核细胞，在体外检测了在虎眼万年青鳞茎中发现的新型22-homo-23-降胆固醇酯苷的生长抑制活性。血液单核细胞（PBMC）。在糖苷的存在下，HL-60细胞和MOLT-4细胞的生长受到强烈抑制。 IC（50）分别为21.0和18.0 nM。糖苷对HL-60细胞生长的抑制作用似乎部分通过诱导细胞凋亡来介导，这通过白血病细胞DNA片段的存在来证明。糖苷处理的HL-60细胞的流式细胞仪分析还显示凋亡细胞的DNA含量低，并且显示G0 / G1细胞减少，S和/或G2 / M细胞随之增加。钙促进了糖苷对HL-60细胞的生长抑制作用，并且在锌的存在下被抑制，这支持了核酸内切酶激活参与糖苷诱导的细胞凋亡。糖苷还抑制了有丝分裂原刺激的PBMC的胚发生，其IC50为6.2 nM。这些结果提供了第一个证据证明万年青甙对人白血病细胞系和PBMC具有有效的生长抑制活性。 [参考：22]

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