目的：观察乳杆菌A2代谢产物LM1和LM2对人口腔舌鳞癌细胞系Cal-27细胞增殖抑制和诱导凋亡作用。方法：将乳杆菌A2经复原乳清培养基培养制备相应的代谢产物1（LM1），除去代谢产物中的钙离子得到产物2（LM2）；用MTT法和流式细胞仪检测口腔舌鳞状细胞癌的细胞凋亡的情况；琼脂糖凝胶电泳检测 DNA 片段的变化。结果：乳杆菌A2代谢产物LM1和LM2作用Cal-27细胞48 h时，最大抑制率分别为（39.9±1.56）%和（30.5±6.85）%；An-nexinv-FITC/PI染色法检测LM2（8 mg/mL）培养40、44 h时，细胞早期凋亡率分别为19.93%和21.42%；琼脂糖凝胶电泳LM2（8 mg/mL）培养54 h时，在250~750 bp之间显现出典型的“梯”状DNA条带。结论：LM1和LM2能抑制口腔舌鳞癌细胞Cal-27的增殖，呈剂量依赖性关系，LM2能够诱导细胞凋亡。%Objective:To investigate the effects of Lactobacillus sp. A2′s metabolites on the proliferation and apoptosis of tongue squamous cell caricoma Cal-27 cells. Method: The recovery of whey Lactobacillus medium (whey) was used to prepare the corresponding metabolites of culture 1 (LM1), and being removed the calcium ions metabolites to give the product 2 (LM2). MTT assay was used to test the inhibition effect of LM2 on Cal-27 cells. AnnexinV-FITC/PI staining flow cytometry was used for cell apoptosis. Agarose gel electrophoresis was used to detect the DNA fragments in Cal-27 cells after the treatment by LM2. Results:After Cal-27 cells were treated with LM1 or LM2 for 48 h, maximum inhibition rates were (39.9%±1.56%) and (30.5%±6.85%) respectively. Flow cytometry analysis with annexinV-FITC/PI showed that after treated with LM2 (8 mg/mL) for 40 h and 44 h, cell apoptosis rate was 19.93%and 21.42%. The typical DNA ladders of the treated cells were detected by agarose gel electrophoresis ,when cultured by LM2 (8 mg/mL) for 54 h, the DNA ladders were between 250~750 bp. Conclusion: LM2 may inhibit the proliferation and induce the apoptosis of Cal-27 cells in a dose-dependent manner.
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