Objective Exploration of the titanium surface nano structure on the cellular bioactivity is of great significance to modifying the pure titanium surface. The unique cluster-like anatase/rutile nanowires (AR@NWs)was prepared and we further examined whether this surface was beneficial for early biological reaction in osteoblast.Methods Unique cluster-like anatase/rutile NWs(AR@NWs)was generated by using a simple hy-drothermal reaction via a three-step synthesis process.The crystal structure of nanowires on titanium surface was de-tected by X-ray diffractometer. The biological activity of nanowires was studied using in vitro cellular experiments. Results The AR@NWs was with a diameter of 200 nm. Three different types of NWs were generated during the production process,displaying different crystal structure and biological characteristics but similar surface topogra-phy and wettability.Compared with sodium titanate NWs(STi@NWs)and H2Ti2O5nanowires(HTi@NWs),AR@NWs surface was conductive to cell attachment. The data indicated that the surface titanium chemical composi-tion and crystal structure played an important role in the cell early response.To some extent,the generation of ana-tase and rutile compensated the cell-repelling properties of NWs. Conclusion Not only the surface physical prop-erties such as surface topography but also the surface chemistry plays an important role in promoting the cell early bioactivity.%目的 探索纯钛表面纳米结构对细胞的生物活性,对纯钛表面改性研究具有重要意义.本实验通过制备独特的簇状锐钛矿/金红石纳米线(AR@NWs)并进一步探讨是否有利于成骨细胞的早期生物学反应.方法 采用水热法制备独特的AR@NWs.主要通过三步合成过程制备,进一步通过X射线衍射仪验证纳米线表面晶相结构,并通过体外细胞实验探索纳米线的生物活性.结果 本实验制备出的纳米线直径约为200 nm.制备过程阶段可以产生3种形貌相似、极具润湿性但晶相不同的纳米线,体现出不同的生物特性.细胞实验中,相对钛酸钠纳米线(STi@NWs)和钛酸纳米线(HTi@NWs),AR@NWs表面利于细胞粘附,显示出表面化学组成和晶相在细胞的早期反应中起着重要的作用,即一定程度上,锐钛矿和金红石的产生可以补偿纳米线的细胞排斥性.结论 纯钛表面除了表面形貌等物理特性,表面化学特性在促进细胞早期生物活性方面也起到非常重要的作用.
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